| 姜黄素对IL-17诱导的人角质形成细胞NO合成以及iNOS表达的影响 |
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| 引用本文： | 杨正生,彭振辉,李晓莉,宋健文,任建文. 姜黄素对IL-17诱导的人角质形成细胞NO合成以及iNOS表达的影响[J]. 细胞与分子免疫学杂志, 2011, 27(9): 959-961 |
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| 作者姓名： | 杨正生 彭振辉 李晓莉 宋健文 任建文 |
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| 作者单位： | 1. 西安交通大学医学院第二附属医院皮肤科,陕西西安710004;秦皇岛市第一医院皮肤科,河北秦皇岛066000
2. 西安交通大学医学院第二附属医院皮肤科,陕西西安,710004 |
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| 基金项目： | 国家自然科学基金资助项目(30371295) |
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| 摘 要： | 目的:探讨姜黄素对IL-17诱导的人表皮角质形成细胞株(HaCaT细胞)NO合成以及诱导型一氧化氮合酶(iN-OS)的mRNA和蛋白表达的影响.方法:用IL-17刺激体外培养的HaCaT细胞,并分别加入3种浓度的姜黄素共培养24h.并收集细胞上清液、提取细胞总RNA、总蛋白,分别进行NO含量的测定、荧光定量PCR和Western blot实验,明确姜黄素对NO含量以及iNOS表达的影响.结果:IL-17能够有诱导HaCaT细胞NO以及iNOS的表达(P＜0.01).姜黄素有效下调NO合成量以及iNOS的mRNA(P ＜0.01)及蛋白表达(P＜0.01)水平.结论:姜黄素对IL-17诱导的HaCaT细胞NO分泌及iNOS的表达具有明显的抑制作用,从而为其对角质形成细胞相关的皮肤炎症性疾病的治疗提供了理论依据.
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| 关 键 词： | 姜黄素 一氧化氮 诱导型一氧化氮合酶 IL-17 角质形成细胞 |
Effect of curcumin on IL-17-induced nitric oxide production and expression of iNOS in human keratinocytes |
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| Yang Zheng-Sheng,Peng Zhen-Hui,Li Xiao-Li,Song Jian-Wen,Ren Jian-Wen. Effect of curcumin on IL-17-induced nitric oxide production and expression of iNOS in human keratinocytes[J]. Chinese journal of cellular and molecular immunology, 2011, 27(9): 959-961 |
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| Authors: | Yang Zheng-Sheng Peng Zhen-Hui Li Xiao-Li Song Jian-Wen Ren Jian-Wen |
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| Affiliation: | YANG Zheng-sheng1,2,PENG Zhen-hui1,LI Xiao-li1,SONG Jian-wen1,REN Jian-wen11Department of Dermatology,Second Affiliated Hospital,Xi'an Jiaotong University,Xi'an 710004,2Department of Dermatology,the First Hospital of Qinhuangdao,Qinhuangdao 066000,China |
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| Abstract: | AIM: To investigate the effect of curcumin on IL-17-induced NO production,mRNA and protein expression of iNOS in human keratinocyte cell lines(HaCaT cells).METHODS: HaCaT cells were stimulated with IL-17 and incubated with three doses of curcumin for 24h in vitro.After collections of supernatant,total RNA and protein,NO levels in supernatant were detected and fluorescence quantitative PCR and Western blot were performed to determine the effect of curcumin on NO levels and iNOS.RESULTS: IL-17 increased NO le... |
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| Keywords: | curcumin nitric oxide inducible nitric oxide synthase IL-17 keratinocyte |
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