RETRACTED ARTICLE: NCL-CD30 staining of epithelial cells in the basal germinative layer of the epidermis and epithelial buds during foetal skin development |
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Authors: | D Tamiolakis N Papadopoulos J Venizelos M Lambropoulou P Tsikouras G Koutsougeras S Bolioti M Tsiapali M Menegaki C Kouskoukis |
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Institution: | (1) Department of Cytology, General Hospital of Chania, Crete, Greece;(2) Department of Histology—Embryology, Democritus University of Thrace, Dragana, 68 100 Alexandroupolis, Greece;(3) Department of Pathology, Ippokration Hospital of Salonica, Salonica, Greece;(4) Department of Obstetrics and Gynecology, Democritus University of Thrace, Alexandroupolis, Greece;(5) Department of Obstetrics and Gynecology, General Hospital of Alexandroupolis, Alexandroupolis, Greece;(6) Department of Dermatology, Democritus University of Thrace, Alexandroupolis, Greece |
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Abstract: | Abstract The fact that the CD30 molecule can mediate signals for cell proliferation or apoptosis prompted us to perform a systematic
investigation of CD30 antigen expression in embryonal tissues during proliferation and differentiation stages. We first targeted
the foetal human intestinal cryptae cells with positive results. The epidermis is a dynamic epithelium that is constantly
renewed throughout life. The basal layer, attached to the basement membrane, contains the dividing cells of the skin and as
cells move up from this layer they undergo differentiation, ending in the formation of a terminally differentiated anucleate
cell called squame. It is intriguing to find out if cells in the basal layer can express the CD30 antigen. We investigated
the immunohistochemical expression of CD30 antigen in 15 paraffin-embedded tissue samples representing epidermis and epidermal
buds from foetuses after spontaneous abortion in the 8th, 10th and 12th weeks of gestation, respectively, using the monoclonal
antibody NCL-CD30. A Northern blotting analysis was additionally performed. The results showed that: (1) the epithelial cells
of the epidermis in the developing skin express the CD30 antigen; (2) CD30 expression in these epithelial cells is higher
in cases of hormonal administration than in normal gestation; (3) a similar positive reaction involved the epidermal buds
associated with the development of the skin appendages. Northern blots of tissue sections using a CD30 cDNA probe detected
mRNAs of the same molecular mass and variety similarly to those in the positive control cell line HUT 102. |
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Keywords: | NCL-CD30 antigen Human foetal skin Gestation Immunohistochemistry Northern blotting |
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