猪腰椎-人颈椎异种椎间盘移植在组织结构和生物力学中的可行性初步研究

目的通过对猪腰椎-人颈椎椎间盘组织结构及生物力学进行对比研究,探讨椎间盘异种移植的可能性。方法采用普通HE和AZAN、Mallory特殊染色。并对特染的组织切片以Tigter图象分析系统进行图象分析,在电子万能实验机上对猪腰椎-人颈椎脊柱试件进行载荷-外移测试同时在脊柱试件施加人体颈椎所承受的极限载荷后观察椎间盘的改变。结果猪腰椎纤维环中弹力纤维所占的面积百分比较人的为多,人颈椎纤维环中胶原纤维的面积百分比较猪的为多,且有显著差异(P<0.01)。髓核中胶原纤维的平均密度,人颈椎椎间盘较猪腰椎椎间盘为大,有显著差异(P<0.01)。椎间盘的载荷-位移曲线近似于S形。其外移均随所受载荷的增大而呈递增关系。猪的腰段脊柱施以人体颈椎所承受的载荷极限值3400N,显微镜下观察猪腰椎椎间盘结构完整。结论猪腰椎椎间盘组织可能成为临床椎间盘损伤修复移植的材料来源。

The histological and biomechanical study on the feasibility of Pig-to-Human xenogeneic intervertebral disc transplantation

Objective To evaluate the feasibility of intervertebral disc xenograft by studying histology and biomechanics of intervertebral disc of pig and human. Methods The annulus fibrous and nucleus pulpous of pig and human were dyed by the methods of H.E,AZAN,Malloy. The dyed tissue was analyzed with the analysis system of tigter. The changes of intervertebral disc were measured with the electronic testing machine while the spinal segement of the human(C2-T1)and pig(T12-S1)were being loaded with the utmost limit of physiology. Results It showed that collagen content in the annulus fibrous of pig increased significantly compared with that of human,But elastic content in the annulus fibrous of pig decreased significantly. The averagy density of collagenic in nucleus pulpous of human increased dramatically compared with that of pig. The type of changed line for intervertebral disc to be loaded with weight was similar to the shape of"S". No changes were found in the intervertebral disc of pig while it was being loaded with the utmost limit of 3400(N). Conclusion The resultes showed that the intervertebral disc of pig can bear against presure force well under physiologic loading. It provide theoretical basis for studying xenogeneic intervertebral disc transplantation from pig to human furtherly.