从分子学角度探讨柴胡-郁金对卒中后抑郁潜在作用机制

目的:基于网络药理学探讨柴胡-郁金药对治疗卒中后抑郁(PSD)的潜在分子学机制。方法:通过检索TCMSP(中药系统药理学技术平台)数据库结合文献挖掘筛选出柴胡-郁金药对的主要活性成分及靶点,采用GeneCard与OMIM数据库获得卒中后抑郁的作用靶点,得到药物与疾病相交的靶点,通过Cytoscape软件建立疾病-药物-成分-靶点网络图,将数据输入String数据库,利用Cytoscape构建靶点相互作用的PPI网络,通过Metascape进行GO和KEGG富集分析。结果:从数据库中筛选了“柴胡-郁金”与PSD的36个共有靶基因和8种共有化合物,PPI网络共涉及节点36个,核心蛋白为IL6、AKT1、VEGFA、CRP、CCL2、MMP9、SERPINE1,GO生物学过程、细胞组成和分子功能分别为39条、8个、11个,KEGG通路共12条。结论:通过网络药理学探讨了“柴胡-郁金”对PSD发挥效应的可能潜在机制,为PSD的进一步研究提供了可能。

To Explore the Potential Mechanism of Chaihu-Yujin on Post-stroke Depression Based on the Molecular Point of View

Objective:To explore the potential molecular mechanism of Bupleurum-Yujin medicine for the treatment of post-stroke depression(PSD)based on network pharmacology.Method:Through searching the TCMSP(Chinese Medicine System Pharmacology Technology Platform)database combined with literature mining to screen out Bupleurum-Yu The main active ingredients and targets of the gold drug pair are used to obtain the targets of post-stroke depression using GeneCard and OMIM database,and the targets where the drug and the disease intersect are obtained.The disease-drug-component-target network diagram is established through the Cytoscape software.The data was input into the String database,Cytoscape was used to construct a PPI network of target interaction,and GO and KEGG enrichment analysis were performed through Metascape.Results:36 shared target genes and 8 shared compounds of“Bupleurum-Yujin”and PSD were screened from the database.The PPI network involved 36 nodes,and the core proteins were IL6,AKT1,VEGFA,CRP,CCL2,MMP9,SERPINE1,GO biological processes,cell composition and molecular functions were 39,8,11,and 12 KEGG pathways.Conclusion:The potential mechanism of“Bupleurum-Yujin”on PSD was explored through network pharmacology,which provided the possibility for further research on PSD.