应用LSCM对脑脊液淋巴白血病细胞、淋巴瘤细胞内质网的研究

目的 :通过激光扫描共聚焦显微镜 (LSCM )技术对脑脊液淋巴白血病细胞 ,淋巴瘤细胞内质网进行立体、动态、定量研究。方法 :将所观察脑脊液细胞分为正常淋巴细胞组、急性淋巴白血病细胞组、鞘注治疗后急性淋巴白血病细胞组、淋巴瘤细胞组。以内质网荧光探针DioC6(3)标记后 ,得到内质网的断层扫描图像、荧光强度地形立体分布图并且定量测量内质网的荧光强度值。结果 :急性淋巴白血病细胞、淋巴瘤细胞与正常淋巴细胞相比 ,鞘注治疗前后淋巴白血病细胞相比 ,内质网空间立体分布有明显差别 ,平均荧光值有非常显著性差异 (P <0 .0 1)。结论 :研究对中枢神经系统淋巴白血病、淋巴瘤的诊断、鉴别诊断、细胞分化程度和功能状态判断有一定意义。

Study on endoplasmic reticulum in cerebrospinal fluid leukemia cells and lymphoma cells by LSCM

Objective:To three-dimensionally, dynamically and quantitatively investigate the endoplasmic reticulum of cerebrospinal fluid leukemia cells and lymphoma cells by laser scanning confocal microscope (LSCM). Methods:Endoplasmic reticulum of all lymphocytes (normal lymphocytes, leukemia cells, cured leukemia cells and lymphoma cells) were stained with fluorescent probe DioC 6 (3), sectional scanograph and fluorescent intensity stereophotography were obtained and finally fluorescent values of cellular endoplasmic reticulum were individually measured. Results:Compared with normal lymphocyte cells, endoplasmic reticulum three-dimensional character of leukemia cells and lymphoma cells were varied and fluorescent value differences were statistically significant (P<0.01). Compared with cured leukemia cells, endoplasmic reticulum three-dimensional character of leukemia cells was varied and fluorescent value differences were statistically significant (P<0.01) too. Conclusion:The experimental works mentioned above may be useful for central nervous system leukemia and lymphoma diagnosis, differential diagnosis, judgement of cells differentiation level and function.