喉鳞状细胞癌组织体外原代培养的初步研究

目的 应用体外培养技术,对喉鳞状细胞癌组织进行体外培养,探讨喉鳞癌组织原代培养中的各种影响因素,为建立人喉鳞癌组织的细胞系提供实验基础。方法采用体外组织培养技术,对24例人喉鳞状细胞癌组织进行原代培养,观察原代培养中肿瘤细胞的生长与供体的年龄、肿瘤组织的分化程度及不同培养方法的关系,分析在人喉鳞癌细胞的培养中成纤维细胞、微生物污染的影响。结果 24例人喉鳞状细胞癌组织标本,年龄小于60岁组的细胞生长率为31.25％(5/16例),年龄大于60岁组为37.5％(3/8例);高分化组为100％(2/2例),中分化组为30.8％(4/13例),低分化组25％(2/8例);组织块培养法为43.75％(7/16例),酶消化法培养为10％(1/10例);倒置显微镜下观察,在培养的第5～7天,在贴壁组织块周围可见到有上皮样细胞爬出。全部标本中,成纤维细胞的过度生长和微生物的污染是阻碍人喉鳞癌细胞生长的重要因素。结论培养组织的细胞生长率与供体的年龄关系不大;肿瘤组织的分化程度较高者,细胞的生长率较高;与酶消化分离培养法相比,贴壁组织块培养法的细胞生长率较高;成纤维细胞及微生物的污染是阻碍人喉鳞癌细胞系建立的重要因素。

A tentative study on primary culture in vitro of twenty-four laryngeal squamous carcinoma tissue

Objective To study the various influ- ent factors on the growth rate of laryngeal carcinoma celi culture in vitro and to provide the experiment base for the establishment of human laryngeal squamous carcinoma celi lines. Methods Applying the technique of primary tissue culture in vitro, twenty-four specimens, which were identified pathologically as laryngeal squa-mous cell carcinoma, were cultured. The relation among the cell growth rate and donor age, the differentiation of the carcinoma cells, cultured methods were observed. The influent factors of primary culture, such as fibrob-last and micro biotic contamination were analyzed. Results Cell growth rate were 31.25% (5/16) inthe group aged under 60, 37.5% in the group above 60, 100% for the well differentiation, 30.8% for the mild differentiation and 25% for the poor differentiation. The cell growth rate were 43.75% in tumor tissue culture and 10% in enzvme digestion culture. The epithelia spread from the tissue in 5(7 days. To all specimens, fibroblast outgrowth and micro biotic contamination were the im-portant factors in obstacle the growth of squamous cell carcinoma. Conclusions The cell growth of laryngeal squamous cell carcinoma in vitro could be correlated with the tumor differentiation and the culture methods; fibroblast outgrowth and micro biotic contamination are important factors to obstacle the establishment of laryn-geal squamous cell carcinoma.