非小细胞肺癌中TMS1基因甲基化检测

目的检测非小细胞肺癌(NSCLC)中TMS1(taget of methylation-induced silencing)基因启动子区域的甲基化及TMS1蛋白表达状况,并研究其临床意义。方法应用甲基化特异性聚合酶链反应和免疫组化的方法检测50例肺癌组织和50例对应癌旁组织中TMS1基因启动子区域甲基化的发生率及TMS1蛋白的表达情况。结果50例NSCLC肿瘤组织中有20例检测到了TMS1基因启动子的甲基化,检出率为40%,相应的癌旁正常组织没有检测到其甲基化(P<0.05)。TMS1蛋白在72%(36/50)的肺癌组织中表达下降,相应的癌旁正常组织均完全表达,没有1例表达下降(P<0.05)。TMS1基因甲基化与TMS1蛋白表达之间有着密切的关系(P<0.05)。结论TMS1基因启动子区域的甲基化是NSCLC的一个常见事件,并且与TMS1蛋白表达下降相关,在NSCLC的发生过程中起着重要的作用。

The methylation status of TMS1 gene in non-small cell lung cancer and its clinical significance

Objective To evaluate the methylation status of taget of methylation -induced silencing gene(TMS1)and protein expression of TMS1 in non-small cell lung cancer(NSCLC),and to explore its clinical significance. Methods The methylation -specific PCR(MSP) and the immunohisto-chemistry(IHC)were used to test the methylation status of DNA and the protein expression of TMS1 from 50 lung cancer tissues and 50 corresponding nomalig-nant tissues. Results 20 of 50 non-small cell lung cancer tissues were tested to be methylated,the frequence is40%,and there was no methylation in any corresponding nomalignant tissue(P<0.05). The protein expression of TMS1 were decreased in 72% of malignant tissues,but were expressed totally in all corresponding nomalignant tissues,none of them was decreased(P<0.05).The methylation status of TMS1 gene was closely correlated with the protein expression of TMS1(P<0.05). Conclusion The methylation of promotor region of TMS1 gene is a common event in non-small cell lung cancer,and it is correlated with the down-regulation of protin expression. The methylation of TMS1 gene plays an important role in tumorigenesis of non-small cell lung cancer.