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| 中间普氏菌内毒素刺激人牙周膜细胞上调表达基因的研究 | |
| 引用本文： | 张贤华,张斌,赵忠良,陈苏民,吴织芬,黄明霞. 中间普氏菌内毒素刺激人牙周膜细胞上调表达基因的研究[J]. 牙体牙髓牙周病学杂志, 2003, 13(6): 311-313 |
| 作者姓名： | 张贤华 张斌 赵忠良 陈苏民 吴织芬 黄明霞 |
| 作者单位： | 1. 四川大学华西口腔医学院,四川,成都,610041 2. 第四军医大学基础部生物化学与分子生物学教研室 3. 第四军医大学口腔医学院,陕西,西安,710032 |
| 摘 要： | 目的：用基因芯片研究中间普氏菌内毒素刺激前后，牙周膜细胞上调表达基因。方法：应用点样数512点的基因芯片分析中间普氏菌内毒素刺激人牙周膜细胞上调表达基因。结果：研究发现中间普氏菌内毒素刺激牙周膜细胞后有6条上调基因，其中部分基因与蛋白激酶和蛋白磷酸酶有关。结论：牙周致病菌内毒素刺激牙周膜细胞后，可引起部分基因表达上调，从而影响牙周膜细胞正常的生理功能。 |
| 关 键 词： | 中间普氏菌 内毒素 基因芯片 牙周膜细胞 |
| 文章编号： | 1005-2593(2003)06-0311-03 |
| 修稿时间： | 2002-03-25 |
Study of Prevotella intermedia LPS on the upregulated gene expression patterns in PDLCs |
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| ZHANG Xian-hua ,ZHANG Bin,ZHAO Zhong-liang,et al. Study of Prevotella intermedia LPS on the upregulated gene expression patterns in PDLCs[J]. Chinese Journal of Conservative Dentistry, 2003, 13(6): 311-313 | |
| Authors: | ZHANG Xian-hua ZHANG Bin ZHAO Zhong-liang et al |
| Affiliation: | ZHANG Xian-hua *,ZHANG Bin,ZHAO Zhong-liang,et al |
| Abstract: | AIM:To analyze the upregulated gene expression patterns in periodontal ligament cells (PDLCs) stimulated by Prevotella intermedia ( Pi ) lipopolysaccharide (LPS) by gene chip and to study the pathogenesis mechanism.METHODS:The upregulated gene expression patterns in PDLCs stimulated by Pi LPS was analyzed by applying gene chip of 512 genes. RESULTS:We identified 6 upregulated genes.Some of them upregulated were involved in protein kinase and protein phosphatase.CONCLUSION:Some PDLCs gene expression were upregulated after stimulated by Pi LPS and the normal physiological function of cells were affected. |
| Keywords: | Prevotella intermedia ( Pi ) lipopolysaccharide (LPS) gene chip periodontal ligament cells (PDLCs) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |