Surface modification of lipid nanocapsules with polysaccharides: From physicochemical characteristics to in vivo aspects |
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| Affiliation: | 1. LUNAM Université, Université d’Angers, INSERM U1066, IBS-CHU Angers, 4 rue Larrey, 49933 Angers, France;2. INSERM-UJF-CRI-U823, Institut Albert Bonniot, 38706 La Tronche, France;1. Departments of Radiology and Radiological Science and Molecular and Comparative Pathobiology, Johns Hopkins University, Baltimore, Maryland;2. Departments of Medicine and Radiology and Medical Imaging, University of Virginia Health System, Charlottesville, Virginia;1. Laboratory of Drug Delivery Technology, Department of Drug Sciences, University of Catania, V.le A. Doria 6, 95125 Catania, Italy;3. Department of Pharmacy, University of Salerno, via Giovanni Paolo II 132, 84084 Fisciano (SA), Italy;1. Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, 15705 Campus Vida, Santiago de Compostela, Spain;2. Pharmacy and Pharmaceutical Technology Department, School of Pharmacy, University of Santiago de Compostela, 15705 Campus Vida, Santiago de Compostela, Spain;3. Radiology Department, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78229-3900, USA;4. Health Research Institute of Santiago de Compostela (IDIS), Santiago de Compostela 15706, Spain;1. EA6295 Nanomédicaments et Nanosondes, Université François Rabelais de Tours, 31 avenue Monge, Tours 37200, France;2. Spincontrol France, 298 rue Giraudeau, Tours 37000, France;3. Bioeurope (Solabia Group), Route d''Oulins, Anet 28260, France;4. Carlina Technologies, 22 rue Roger Amsler, Angers 49100, France;1. Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre 90610-000, Brazil;2. Programa de Pós-Graduação em Ciências Biológicas: Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035 000, Brazil;3. Programa de Pós-Graduação em Odontologia, Faculdade de Odontologia, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-003, Brazil;4. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-003, Brazil;5. Food and Drug Department, University of Parma, Parco Area delle Scienze 27/a, 43124 Parma, Italy;6. Interdepartmental Centre for Innovation in Health Products - Biopharmanet-TEC, University of Parma, Padiglione 33, Campus Universitario, 43124 Parma, PR, Italy;1. Faculty of Pharmacy, Université de Montréal, C.P. 6128, succursale Centre-ville, Montréal (Québec) H3C 3J7, Canada;2. Univ Angers, Inserm, CNRS, MINT, SFR ICAT, F-49000 Angers, France;3. INRS Centre Armand-Frappier Santé Biotechnologie, 531 Boul. des Prairies, Laval (Québec) H7V 1B7, Canada;4. Univ Angers, Plateforme d''Analyse Cellulaire et Moléculaire (PACeM), SFR ICAT, F-49000 Angers, France |
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| Abstract: | Attaching polysaccharides to the surface of nanoparticles offers the possibility of modifying the physicochemical and biological properties of the core particles. The surface of lipid nanocapsules (LNCs) was modified by post-insertion of amphiphilic lipochitosan (LC) or lipodextran (LD). Modelling of these LNCs by the drop tensiometer technique revealed that the positively charged LC made the LNC surface more rigid, whereas the neutral, higher MW LD had no effect on the surface elasticity. Both LNC-LC and LNC-LD activated the complement system more than the blank LNC, thus suggesting increased capture by the mononuclear phagocyte system. In vitro, the positively charged LNC-LC were more efficiently bound by the model HEK293(β3) cells compared to LNC and LNC-LD. Finally, it was observed that neither LC nor LD changed the in vivo biodistribution properties of LNCs in mice. These polysaccharide-coated LNCs, especially LNC-LC, are promising templates for targeting ligands (e.g. peptides, proteins) or therapeutic molecules (e.g. siRNA). |
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