米非司酮对宫颈癌Caski细胞凋亡的影响

目的研究米非司酮对体外人宫颈鳞癌Caski细胞生长抑制情况，为临床应用米非司酮治疗宫颈鳞癌提供实验依据。方法体外培养人宫颈鳞癌Caski细胞，应用不同浓度的米非司酮处理，采用流式细胞术观察各组细胞凋亡率，并分析细胞周期的变化；异硫氰酸荧光素（FITC）荧光标记流式细胞术（FCM）法测定米非司酮对Caski细胞HPV—E6、p53、Bcl-2和Bax的表达和活性变化。结果流式细胞术结果显示12．5mg／L以上浓度的米非司酮能明显诱导Caski细胞凋亡，使细胞周期阻滞于G1期，呈明显的浓度-时间依赖方式，而1．25mg／L浓度对Caski细胞无明显的诱导凋亡作用；FITC荧光标记FCM法结果显示米非司酮作用于Caski细胞后。HPV—E6、Bcl-2蛋白表达下调，p53、Bax蛋白表达上调，呈浓度依赖方式（P〈0．05）。结论大剂量米非司酮（12．5～20mg／L）具有诱导Caski细胞凋亡作用，能使其周期阻滞于G1期，与下调HPV16-E6、Bcl-2蛋白表达，上调p53、Bax蛋白表达有关。

Effect of mifepristone on the apoptosis of human cervical squamous carcinoma cells

Objective To investigate the inhibitory effect of mifepristone the growth of human cervical squamous carcinoma ceils in vitro and explore the mechanism of mifepristone. Methods Human cervical squamous carcinoma ceil line Caski ceils were cuhuled in vitro, Caski ceils was treated with various concentration of mifepristone. The apoptosis of Caski cells was determined by PI staining flow cytometry and ceil cycle was analyzed using flow cytometry;The expression and variation of activity of HPV16 -E6, p53, Bcl -2, Bax protein in Caski cells were investigated by FITC staining flow cytometry. Results The apoptosis of Caski cells induced and ceil cycle was arrested at G1 phase by mifepristone whose concentrateion excessed 12.5mg/L in a concentrateion and time dependent manner, The apoptosis of Caski ceils can not be induced by mifepristone at concentrateions of 1.25mg/L. FITC staining flow cytometry indicated the expression of HPV16 - E6,Bcl - 2 protein decreased and the expression of p53 and Bax protein, increased in Caski ceils treated with mifepristone in a concentrateion dependent manner （P 〈 0.05）. Conclusion Large dose of mifepristone （ 12.5 - 20.0mg/L） can significantly induce the apoptosis of human cervical squamous carcinoma Caski ceils, arrest ceil cycle in G1 phase. The effect of mifepristone on apoptosis of Caski ceils is associated with downregulating expression of HPVI6 - E6 ,Bcl - 2 protein and upregulating expression of p53 and Bax protein.