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Secretion and accumulation of Alzheimer's β-protein by cultured vascular smooth muscle cells from old and young dogs |
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| Authors: | Janusz Frackowiak Bozena Mazur-Kolecka Henryk M Wisniewski Anna Potempska Richard T Carroll Mark R Emmerling Kwang Soo Kim |
| Institution: | aDepartment of Pathological Neurobiology, New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY 10314, USA;bDepartment of Molecular Biology, New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY 10314, USA;cDepartment of Virology, New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY 10314, USA;dNeurodegenerative Diseases Group, Parke-Davis Pharmaceutical Research Division, 2800 Plymouth Road, Ann Arbor, MI, USA;eVisiting scientist from Department of Pathophysiology, Medical School, Gdansk, Poland;fVisiting scientist from Department of Immunobiology, Institute of Pharmacology, Polish Academy of Sciences, Cracow, Poland |
| Abstract: | Cultured smooth muscle cells isolated from β-amyloid-affected blood vessels from old dogs accumulate β-protein at early passages 5,24]. Now, we show that smooth muscle cells derived from amyloid-free brain blood vessels and peripheral arteries from old and young animals are induced by culture conditions to deposit intracellularly fibrillar and non-fibrillar β-protein. Accumulation of β-protein is associated with a higher secretion of β-protein, but not with a higher secretion of β-amyloid precursor protein (βAPP) or higher cellular content of βAPP. Gradual cessation of proliferative activity was observed in cultures that accumulate β-protein. |
| Keywords: | Smooth muscle cell β -Protein β -Amyloid β -Amyloid precursor protein Tissue culture |
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