| PURIFICATION AND CHARACTERIZATION OF A TUMOR-ASSOCIADET HIGH MOLECULAR WEIGHT SERUM DNA BINDING PROTEIN |
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| 引用本文： | 龚秋明,童坦君. PURIFICATION AND CHARACTERIZATION OF A TUMOR-ASSOCIADET HIGH MOLECULAR WEIGHT SERUM DNA BINDING PROTEIN[J]. 北京大学学报(医学版), 1991, 0(1) |
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| 作者姓名： | 龚秋明 童坦君 |
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| 作者单位： | Department of Blochemistry，Beijing Medical University，Department of Blochemistry，Beijing Medical University |
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| 摘 要： | A high molecular weight DNA binding protein (HDBP) was separated fromserum DNA binding proteins (DBPs) of mice with Ehrlich ascites carcinoma by immunoa-dsorption and immunoaffinity chromatography. HDBP reacted with antiserum against DBPsfrom mice with Ehrlich ascites carcinoma to form a precipitation band, but did not reactwith antiserum against DBPs from normal mice in Ouchterlony double diffusion studies.Apparent molecular weights of reduced and nonreduced HDBP were 41 000 and 280 000respectively, based upon SDS-polyacrylamide gel electrophoresis. HDBP gave positive reacti-on in periodic acid Schiff's glycoprotein staining method. In isoelectric focusing analysis, theisoelectric point of HDBP was in a range between 6.4 and 6.5. The amino acid compositionof HDBP was rich in valine and hydrophilic amino acids such as glutamic acid, threonineand serine.
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PURIFICATION AND CHARACTERIZATION OF A TUMOR-ASSOCIADET HIGH MOLECULAR WEIGHT SERUM DNA BINDING PROTEIN |
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| Gong Qiuming,Tong Tanjun. PURIFICATION AND CHARACTERIZATION OF A TUMOR-ASSOCIADET HIGH MOLECULAR WEIGHT SERUM DNA BINDING PROTEIN[J]. Journal of Peking University. Health sciences, 1991, 0(1) |
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| Authors: | Gong Qiuming Tong Tanjun |
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| Affiliation: | Gong Qiuming,Tong Tanjun Department of Blochemistry,Beijing Medical University |
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| Abstract: | A high molecular weight DNA binding protein (HDBP) was separated fromserum DNA binding proteins (DBPs) of mice with Ehrlich ascites carcinoma by immunoa-dsorption and immunoaffinity chromatography. HDBP reacted with antiserum against DBPsfrom mice with Ehrlich ascites carcinoma to form a precipitation band, but did not reactwith antiserum against DBPs from normal mice in Ouchterlony double diffusion studies.Apparent molecular weights of reduced and nonreduced HDBP were 41 000 and 280 000respectively, based upon SDS-polyacrylamide gel electrophoresis. HDBP gave positive reacti-on in periodic acid Schiff's glycoprotein staining method. In isoelectric focusing analysis, theisoelectric point of HDBP was in a range between 6.4 and 6.5. The amino acid compositionof HDBP was rich in valine and hydrophilic amino acids such as glutamic acid, threonineand serine. |
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| Keywords: | Carcinoma DNA binding protein Serum protein Purification |
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