| 创造酶切位点PCR-RFLP检测PON2(rs12026)基因多态性 |
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| 引用本文： | 王威,姚武,周舫,李春阳,燕贞,吴拥军,吴逸明.创造酶切位点PCR-RFLP检测PON2(rs12026)基因多态性[J].河南医学研究,2010,19(3). |
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| 作者姓名： | 王威 姚武 周舫 李春阳 燕贞 吴拥军 吴逸明 |
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| 作者单位： | 郑州大学公共卫生学院劳动卫生与毒理学教研室,河南,郑州,450001 |
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| 基金项目： | 国家自然科学基金，河南省教育厅自然科学研究计划项目 |
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| 摘 要： | 目的:建立简便易用的PON2基因单核苷酸多态(rs12026)的检测方法。方法:应用创造酶切位点原理设计引物,通过PCR-RFLP技术判断PON2基因SNP位点多态性。结果:设计一对特异引物并PCR扩增含PON2多态位点的DNA片段,根据限制性内切酶BsmAI酶切结果判断PON2位点多态性,PCR和酶切效果均较好。结论:应用创造酶切位点PCR-RFLP原理建立的PON2多态位点的检测方法具备简便、经济、快速的特点。
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| 关 键 词： | 创造酶切位点 聚合酶链反应-限制性酶切片段多态性 单核苷酸多态性 PON2 引物设计 |
Application of CRS-PCR-RFLP to identify PON2 (rs12026) gene polymorphisms |
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| WAGN Wei,YAO Wu,ZHOU Fang,LI Chun-yang,YAN Zhen,WU Yong-jun,WU Yi-ming.Application of CRS-PCR-RFLP to identify PON2 (rs12026) gene polymorphisms[J].Henan Medical Research,2010,19(3). |
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| Authors: | WAGN Wei YAO Wu ZHOU Fang LI Chun-yang YAN Zhen WU Yong-jun WU Yi-ming |
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| Abstract: | Objective: To develop a proper assay for identifying single nucleotide polymorphism(SNP) of PON2 gene.Methods: PCR primers were designed by create restriction site(CRS) method,then PCR-RFLP was adopted to identify the SNP in PON2 gene.Results: One primer pair can get target products containing PON2 SNP site by PCR,restriction enzymes BSMAI were adopted to identify the SNP site.The results of PCR and RFLP were good.Conclusion: It suggested that the method of detecting the SNP of PON2 by CRS-PCR-RFLP theory was facility,economy,and rapid. |
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| Keywords: | PON2 |
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