| 儿童肺炎链球菌对三种抗菌药物的耐药性及相关耐药基因的研究 |
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| 引用本文： | 余晓君,王晔,应蕾. 儿童肺炎链球菌对三种抗菌药物的耐药性及相关耐药基因的研究[J]. 江西医学检验, 2008, 26(1): 15-17 |
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| 作者姓名： | 余晓君 王晔 应蕾 |
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| 作者单位： | 余晓君（330006,南昌,江西省儿童医院）;王晔（330006,南昌,江西省儿童医院）;应蕾（330006,南昌,江西省儿童医院） |
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| 摘 要： | 目的了解南昌地区儿童肺炎链球菌（streptococus pnenurmoniae，SP）对青霉素、红霉素、四环素耐药状况。方法对呼吸道、血液标本、脑脊液标本中分离的31株SP进行青霉素、头孢噻肟E-test药敏试验及青霉素、红霉素和四环素耐药相关的PBP2B、ermB、ermA／B／C、mefA和Term基因PCR检测。结果①PBP2B基因突变30株，均为点突变，PBP2B同源性在80。85％之间。另有1株对青霉素敏感无突变；②含ermA／B／C耐药基因24株，含InerA耐药基因26株，含有erm或InefA耐药基因30株。结论PBP2B基因突变在对青霉素耐药的同时也对三代头孢表现为不同程度的耐药．SP的耐药性已严重影响青霉素．红霉素．四环素的疗效。
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| 关 键 词： | 肺炎链球菌 青霉素 耐药基因 |
| 文章编号： | 1674-1129(2008)01-0015-03 |
Detection of three antimicrobial resistance and resistant gene from streptococcus pneumoniae |
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| YU Xiaojun,WANG Ye,YING Lei. Jiangxi provincial Children Hospital,Nanchang ,China. Detection of three antimicrobial resistance and resistant gene from streptococcus pneumoniae[J]. Jiangxi Journal of Medical Laboratory Sciences, 2008, 26(1): 15-17 |
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| Authors: | YU Xiaojun WANG Ye YING Lei. Jiangxi provincial Children Hospital Nanchang China |
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| Affiliation: | YU Xiaojun,WANG Ye,YING Lei. Jiangxi provincial Children Hospital,Nanchang 330006,China |
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| Abstract: | Objective To investigate the relation of streptococcus pneumoniae(SP) and their resistance to penicillin,erythromycin and tetracysline. Methods With thirty-one strains of Sp isolated from respiratory tract secretion,blood and cerebrospinal fluid,the susceptibilities of the strains to penicillin and cefotoxine was determined by E-tetst. The genes expressions of PBP2BermB,ermA/B/C,mefA and TetM,related resistance to penicillin,erythromycin and cyclomycin,were detected by PCR. Results PBP2B gene point mutation... |
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| Keywords: | Streptococcus pneumoniae Drug resistance gene |
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