丹参素对肝星状细胞ERK1/2信号转导通路的影响

目的:观察丹参素对血小板衍生生长因子-BB(Platelet derived growth factor-BB,PDGF-BB)刺激大鼠肝星状细胞(Hepatic stellate cell,HSC)增殖、Ⅰ型胶原合成、磷酸化细胞外信号调节激酶1,2(Phosphoryhted extracellular signal-regulated kinase 1/2,P-ERK1/2)表达的影响,探讨丹参素抗肝纤维化的分子机制.方法:四甲基偶氮唑盐法检测大鼠HSC增殖活性;免疫细胞化学染色法测定大鼠HSC Ⅰ型胶原合成的表达;Western blot测定大鼠肝星状细胞P-ERK1/2的表达.结果:丹参素有抑制PDGF-BB刺激大鼠HSC增殖,Ⅰ型胶原表达,p-ERK1/2表达的作用.结论:丹参素可抑制PDGF-BB刺激的大鼠HSC增殖,Ⅰ型胶原合成,其机制可能与抑制ERK1/2信号转导通路有关.

Effect of Danshengsu on ERK1/2 signal transduction pathway in hepatic stellate cell

Objective:To investigate the inhibitory effect of Danshengsu on proliferation, collagen type Ⅰ synthesis and Phosphorylated extracellular signal- regulated kinase 1/2 (P-ERK1/2)expression in rat hepatic stellate cell (HSC) stimulated by platelet derived growth factor-BB (PDGF-BB), and to elucidate the molecular mechanism of Danshensu against hepatic fibrosis: Methods: Cell proliferation was evaluated by 3-(4,5-dimethylthiazol-2-yl )-2,5-Diphennyltetrazolium bromide(MTT)colorimetric assay.Collagen type Ⅰ synthesis was detected by Immunocytochemical stain.Phosphorylated extracellular signal- regulated kinase 1/2 (P-ERK1/2) expression was detected by Western blot. Results:Danshensu could inhibit proliferation,collagen type Ⅰ synthesis and P-ERK1/2 expression of HSC stimulated by PDGF-BB. Conclusion:Danshensu can inhibit proliferation and Collagen type Ⅰ synthesis of HSC stimulated by PDGF-BB,the mechanism of which may be Danshensu, s inhibitory effect on ERK1/2 signal transduction pathway.