差异表达基因Hepsin在前列腺癌中的表达及其意义

目的探讨cDNA芯片筛选出的前列腺癌（PC）差异表达基因Hepsin在PC中的表达及其意义。方法应用RT—PCR检测Hepsin在正常前列腺组织、PC组织及PC细胞系中的表达，并进行半定量分析，同时采用免疫印迹法检测Hepsin的表达，采用免疫组织化学染色法检测40例PC标本、15例前列腺增生（BPH）标本和6例正常前列腺标本的Hepsin表达水平。结果在mRNA水平，Hepsin在PC中的表达水平高于正常前列腺组织（P=0．026），但在PC细胞系中的表达并不增高。在蛋白表达水平，正常前列腺组织未表达Hepsin，而PC及PC细胞系均表达Hepsin。PC和BPH标本免疫组化检测均为阳性，但两者间有非常高度显著性差异（P=0．000），而正常前列腺标本为阴性。结论Hepsin在PC中的表达增高，有可能作为早期诊断的标志以及基因治疗的靶基因。

Expression and Significance of Differential Expression Gene Hepsin in the Prostate Cancer

Objective To evaluate the expression and significance of differential expression gene Hepsin in the prostate cancer (PC) screened by the cDNA microarray technique.Methods The techniques of semiquantitative RT-PCR and Western blotting were used to detect the mRNA and protein expression of Hepsin. Specimens of 40 cases of PC, 15 benign prostatic hyperplasia (BPH) and 6 normal prostate tissues were examined by the immunohistochemical stain.Results Hepsin was more expressed in PC tissue than normal prostate tissue ( P=0.026) and was confirmed by Western blot analysis. Immunohistochemical test confirmed this and demonstrated that Hepsin did not expressed in normal prostate but expressed in PC and BPH and there was a significant difference in Hepsin expression level between PC and BPH tissues ( P=0.000).Conclusion Hepsin high expressed in PC may be a new molecular marker in early diagnosis of PC and a new target for gene therapy of PC.