抗一HCV阳性无病毒血症患者HCV特异性免疫反应研究

目的 研究抗ＨＣＶ阳性无病毒血症患者特异的体流及细胞免疫状态。方法 对１５例抗ＨＣＶ阳性无病毒血症患者，１５例慢性ＨＣＶ持续感染者及１５例正常对照者进行研究。通过ＭＴＴ、ＬＤＨ释放法、酶联免疫等方法观察患者外周Ｔ细胞反应、自然杀伤（ＮＫ）细胞活性、细胞因子分泌及特异性体液免疫反应。结果 无病毒血症者Ｔ细胞增殖反应明显大于持续感染者及对照组，并且Ｔ细胞对核心区抗原增殖最大，ＮＳ３次之，对ＮＳ５则无明

Study of hepatitis C virus specific immuine responses in anti- HCV positive patients without hepatitis Cviremia

OBJECTIVE: To study the hepatitis C virus specific immune responses in anti - HCV positive patients without hepatitis C viremia. METHODS: 15 anti-HCV positive patients without hepatitis C viremia, 15 patients with chronic HCV infection and 15 normal controls were selected for this study. The T cell responses, NK cell (natural killer cells) activity, cytokine production and HCV specific antibodies were detected by MTT, LDH release and ELISA. RESULTS: Our study showed that the T cell proliferative reaction of patients without hepatitis C viremia was significantly higher than that of patients with chronic HCV infection and normal controls and the T cell response for HCV core antigen were higher than NS3 and N54, but there was no significant proliferative response to NS5 antigen. We also found that there were no differences in anti-HCV antibody production and NK cell activity between the two groups and the level of IFN-gamma in patients without hepatitis C viremia was higher than that in patients with persistent HCV infection. CONCLUSIONS: There are a lot of advantageous changes of HCV specific humoral and cellular immune response in anti-HCV positive patients without hepatitis C viremia, these immune responses may play a role in clearance of HCV.