Inhibition of interferon-alpha/beta induction in L-929 cells by benzene and benzene metabolites |
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Authors: | S C Cheung D E Nerland G Sonnenfeld |
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Affiliation: | Department of Microbiology and Immunology, School of Medicine, University of Louisville, Ky. |
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Abstract: | Murine L-929 cells were treated with benzene or a series of benzene metabolites, washed and then interferon-alpha/beta was induced with polyriboinosinic-polyribocytidylic acid. Exposure of the cells to benzene or phenol, a monocyclic metabolite of benzene, did not affect interferon-alpha/beta induction. However, exposure of the cells to p-benzoquinone, hydroquinone or catechol, dihydroxy- and diketo-metabolites of benzene, resulted in a severe inhibition of interferon-alpha/beta production. There was no significant loss of viability of the cell cultures. Additional studies with p-benzoquinone indicated that inhibition of interferon-alpha/beta was reversible and could be abrogated by addition of reduced glutathione to the cell cultures. |
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