Rapid and simple isolation of DNA from agarose gels |
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Authors: | Martin Grey Martin Brendel |
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Institution: | (1) Institut für Mikrobiologie der J. W. Goethe-Universität, Theodor-Stern-Kai 7, Haus 75, W-6000 Frankfurt/Main, Federal Republic of Germany |
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Abstract: | Summary We present a simple method for the isolation of DNA from agarose gels that is economic, fast, and independent of electrical equipment. DNA fragments of up to 6 kb can be easily extracted within 5 min using a disposable plastic syringe and filter paper. Total extraction of DNA fragments between 10 and 20 kb in size is achieved by concentrating the DNA flushed from the gel in a DNA-binding column. |
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Keywords: | DNA isolation Agarose gels Molecular cloning |
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