散发性大肠管状腺瘤癌变过程中STAT3和p38的表达及意义

目的探讨STAT3和p38在散发性大肠管状腺瘤癌变过程巾的可能作用。方法采用免疫组化方法检测107例散发性大肠管状腺瘤伴不同程度异型增生及癌变组织中STAT3和p38的蛋白表达水平,采用原位杂交方法检测STAT3 mRNA的表达。同时选取25例正常大肠黏膜作为对照。结果STAT3蛋白在正常大肠黏膜、大肠管状腺瘤伴上皮异型增生和癌变组中的阳性表达率分别为12.0%、59.0%和91.7%,STAT3 mRNA的阳性表达率分别为8.0%、51.8%和100.0%,p38蛋白的阳性表达率分别为8.0%、47.0%和91.7%。大肠管状腺瘤伴上皮异型增生组和癌变组STAT3蛋白、STAT3 mRNA和p38蛋白的阳性表达率均明显高于正常大肠黏膜组(P均<0.05),而癌变组均高于大肠管状腺瘤伴上皮异型增生组(P均<0.05)。大肠管状腺瘤伴上皮异型增生患者中,STAT3蛋白、STAT3 mRNA和p38蛋白的阳性表达率均随异型增生程度增高而增高(P<0.05)。STAT3与p38的表达呈正相关(r=0.515)。结论STAT3和p38均可能在散发性大肠管状腺瘤癌变过程中起一定作用。

Expressions and significance of STAT3 and p38 in the carcinogenesis of sporadic colorectal tubular adenoma

Objective To investigate the possible role of STAT3 and p38 in the carcinogenesis of sporadic colorectal tubular adenoma. Methods The expression of STAT3 and p38 at protein level was studied in 107 sporadic colorectal tubular adenomas with different dysplasia ( SCTA-D) or with cancerous changes(SCTA-Ca) by immunohistochemical staining method, meanwhile the expression of STAT3 at mRNA level was detected by in situ hybridization. Results Immunohistochemical staining results showed that the positive expression rate of STAT3 and p38 was 12. 0% , 59. 0% , 91.7% and 8. 0% ,47. 0% , 91.7% in normal colorectal mucosa ( NCM ), SCTA-D and SCTA-Ca, respectively, with a statistically significant difference of STAT3 and p38 expression among the SCTA-D, SCTA-Ca and NCM ( P < 0. 05 ). The expression of STAT3 and p38 was positively correlated with the degree of dysplasia from mild to severe SCTA-D (P < 0. 05 ). In situ hybridization results showed that the positive expression rate of STAT3 at mRNA level in NCM, SCTA-D and SCTA-Ca was 8. 00% , 51. 8% and 100. 0% , respectively, with a statistically significant difference among these either ( P < 0. 05). The positive expression of STAT3 at mRNA level was not only positively correlated with the degree of dysplasia (P < 0. 05) , but also with the expression of p38 (P < 0. 05). Conclusion STAT3 and p38 may be involved in the carcinogenesis of sporadic colorectal tubular adenoma.