环氧合酶-2和基质金属蛋白酶-9在宫颈癌中的表达及其临床意义

目的研究环氧合酶-2(COX-2)和基质金属蛋白酶-9(MMP-9)在宫颈癌组织中的表达及临床意义。方法采用免疫组化S-P法检测72例宫颈浸润癌(ICC)、16例癌旁正常宫颈上皮(NCE)组织中COX-2和MMP-9的表达,并分析COX-2和MMP-9表达与ICC病理分级、临床分期、间质浸润和淋巴结转移等临床病理特征的关系。结果ICC组织中COX-2和MMP-9的表达显著高于NCE组织,72例ICC中,COX-2阳性表达率为88.9%,MMP-9阳性表达率为94.4%;16例NCE组织中,COX-2阳性表达率为12.5%,MMP-9阳性表达率为43.8%,两组间差异均有统计学意义(P< 0.01)。COX-2表达与ICC的淋巴结转移和间质浸润深度呈正相关(r分别为0.296和0.257)。MMP-9的表达与ICC的临床分期、病理分级呈正相关(r分别为0.329和0.351)。COX-2和MMP-9在宫颈癌组织中的表达强度呈正相关(r=0.297),二者表达的一致率高达86.1%(62/72)。结论COX-2和MMP-9的高表达与宫颈癌的侵袭和发展有关,二者在宫颈癌的细胞增殖、浸润和转移中有协同作用,联合检测二者的表达对于进一步理解宫颈癌的生物学行为和判断预后有一定价值。

Expression of COX-2 and MMP-9 in cervical carcinoma and their clinical significance

Objective To investigate the expressions of COX-2 and MMP-9 in cervical carcinoma and their clinical significance. Methods Immunohistochemical SP method was used to detect the expressions of COX-2 and MMP-9 in 72 cases of invasive carcinoma of cervix ( ICC) and 16 cases of normal cervical epithelium remote from tumor (NCE) , and the relationship between the expressions of COX-2 and MMP-9 in ICC and some factors relating to clinical pathology of cervical carcinoma such as histopathological grading, lymph node metastasis, stroma involvement and FIGO staging were analyzed statistically. Results The rates of positive expression of COX-2 and MMP-9 in ICC were significantly higher than those in NCE. COX-2: 88.9% in group ICC and 12.5% in group NCE, P = 0.000. MMP-9: 94.4% in group ICC and 43.8% in group NCE, P = 0. 000. The expression of COX-2 was positively correlated with lymph node metastasis and stroma involvement (r =0. 296, P =0. 012 in group ICC and r =0. 257 , P =0. 029 in group NCE, respectively). The expression of MMP-9 was positively correlated with FIGO staging (r = 0. 329, P = 0. 005) and histopathological grading ( r = 0. 351, P = 0. 003 ). The expression of COX-2 was positively correlated with the expression of MMP-9 in ICC (r = 0. 297,P = 0.011). Conclusion The overexpressions of COX-2 and MMP-9 are closely related to the invasion and growth of cervical carcinoma. The tissue with overexpression of COX-2 has strong invasion ability. COX-2 and MMP-9 have synergistic effect on proliferation, invasion and metastasis of cancer cells. Detecting the expression of both COX-2 and MMP-9 may be of value in further understanding the biological behavior and predicting the prognosis of cervical carcinoma.