激光照射与酸性氟磷酸盐联合应用对离体人牙齿釉质脱矿的影响

目的 :研究激光照射及酸性氟磷酸盐 (acidulatedphosphatefluoride ,APF)处理离体人牙齿釉质 ,对牙釉质抗酸性的影响。方法 :10 0、15 0、2 0 0mJ 3种能量的激光照射光滑面及咬合面牙釉质后用 12 .3g/LAPF处理 ,对照组仅用 12 .3g/LAPF处理。将处理过的牙齿置于人工龋液中脱矿 ,测量不同时间后脱矿液中的釉质钙溶出量 ;偏光显微镜下测量人工龋的深度。结果 :酸蚀 10h后 ,实验组釉质钙溶出量少于对照组 (P <0 .0 5 ) ;酸蚀 2 4h后各组之间的钙溶出量无显著的统计学差异 (P >0 .0 5 )。 10 0mJ激光照射咬合面组的人工龋损深度少于其他 3组 (P <0 .0 5 ) ,但 15 0、2 0 0mJ激光照射组与对照组相比无显著的统计学意义 (P >0 .0 5 )。结论 :10 0mJ激光照射后再使用 12 .3g/LAPF可明显增强光滑面及咬合面牙釉质的抗酸性

The effect of Nd:YAG laser irradiation combined with acidulated phosphate fluorid treatment on the acid resistance of human enamel

Objective: To study the effect of pulsed Nd:YAG laser irradiation combined with acidulated phosphate fluorid (APF) on the acid resistance of human dental enamel. Methods: Enamel samples were prepared from 192 caries-free extracted teeth. The smooth surface and pits and fissures were treated with 12 3 g/L APF for 4 minutes after 100,150 or 200 mJ pulsed Nd:YAG laser irradiation. Then the teeth were put into 12.3 g/L of artificial caries inducing solution (acid solution) for 1,5,10 or 24 h respectively. The control teeth were treated with APF only. The amount of calcium dissolved in the solution was determined with an atomic absorption spectrophotometer and the depth of artificial caries was measured with polarized microscope. Results: The amount of calcium dissolved in the acid solution was significantly less in the groups with laser treatment than that in those without laser treatment (P<0.05) after 10 hours etching. The lesion depth of the teeth with 100 mJ laser irradiation and APF treatment was less than that of others(P<0.05). Conclusion:Laser irradiation combined with APF treatment can increase the acid resistance of enamel.