胃癌P53基因突变和蛋白表达的研究 |
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引用本文: | 刘孟珉 余龙. 胃癌P53基因突变和蛋白表达的研究[J]. 癌变.畸变.突变, 1997, 9(5): 271-274 |
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作者姓名: | 刘孟珉 余龙 |
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作者单位: | [1]上海第二军医大学附属长征医院实验科 [2]复旦大学遗传学研究所 |
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摘 要: | 为了解中国人胃癌P53基因突变情况,应用银染聚合酶链反应-单链构象多态(PCR-SSCP)技术对P53基因第5、6、7、8外显子进行检测。对来自癌组织DNA和癌旁正常组织DNA的PCR-SSCP电泳带迁移作对比分析,发现37例胃癌中,11例胃癌样品电泳带迁移异常,依据DNA单链构象与分子电泳迁移的关系,研究结果表明:胃癌P53基因5-8外显子的突变率为30%。微波处理ABC法检测突变型P53蛋白表
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关 键 词: | 胃肿瘤 癌 P53基因 突变 蛋白 表达 |
A STUDY OF P53 GENE MUTATION AND PROTEIN EXPRESSION IN HUNAN GASTRIC CANCER |
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Abstract: | Silver staining PCR-SSCP method was used to detected the mutation of p53 gene exon5,6,7 and 8.Mobility of PCR amplification product from tumor tissue DNA was compared with that of para-cancer normal tissue DNA by PCR-SSCP .Mobility shife were observed in 11 out of 37 cases of gastric cancer.The date on relationship between mobility and single strand conformation showed that the mutation rate in exon 5,6,7 and 8 from these cancer cases was 30%.The mutant p53 protein was detected by microwave oven treatment and ABC immunohistochemistry,its positive nuclear staining was observed in 17 out of 37 cases of gastric cancer (46%).It is suggested that mutation of p53 gene,which result in abnormal protein accumulation,was a frequent molecular structural change and may have importance in carcinogenesis and progression of gastric cancer. |
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Keywords: | Gastric cancer p53 gene Mutation PCR-SSCP Immunohistochemistry |
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