DADLE对大鼠急性全脑缺血再灌注后海马区神经元ERK信号通路和Caspase-3表达的影响 |
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引用本文: | 洪妙玲,黄伟青,刘佩仪,武钊,文锐玲,郭兰英,黄瑞娇,欧阳斌,郭伟,梁庆.DADLE对大鼠急性全脑缺血再灌注后海马区神经元ERK信号通路和Caspase-3表达的影响[J].现代保健,2013(2):7-9. |
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作者姓名: | 洪妙玲 黄伟青 刘佩仪 武钊 文锐玲 郭兰英 黄瑞娇 欧阳斌 郭伟 梁庆 |
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作者单位: | 广州医学院第一附属医院,广东广州510120 |
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基金项目: | 基金项目:2011年广州市大学生科技创新实践项目(编号:1057011004);广州市医药卫生科技项目(编号:201102A213152) |
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摘 要: | 目的:观察8-阿片受体激动剂DADLE(D—Ala2,D—Leu5]enkephali)对大鼠急性全脑缺血再灌注后海马区神经元ERK信号通路和Caspase一3表达的影响。方法:将50只sD大鼠随机分为5组:假手术组(Sham)、模型组(I/R)、DADLE处理组(根据不同剂量可分为2mg/kgA]、3mg/kgB]、5mg/kgC]o采用改良的二血管阻断加低血压法建立全脑缺血再灌注模型。于缺血15min后经颈外静脉注射DADLE并于再灌注120min后处死。开颅取其新鲜海马组织,采用western blot检测海马组织Caspase-3的表达,以及采用免疫组织化学法检测非磷酸化ERK与磷酸化ERK(P—ERK)的表达状况。结果:Sham组ERK和P—ERK蛋白表达水平显著低于其他各组(P〈0.05),DADLE处理组与I/R组相比,其ERK和P—ERK蛋白的表达量明显上调(P〈0.05)。海马组织Caspase-3蛋白表达I/R组较Sham组表达明显上调(P〈O.05),而DADLE处理组与IR组比较,海马组织Caspase-3蛋白表达明显下降(P〈0.05)。结论:DADLE对大鼠急性全脑缺血再灌注后海马区神经元有保护作用,说明DADLE可通过上调ERK信号通路以及抑制Caspase-3的表达而起到保护脑组织的作用。
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关 键 词: | DADLE 全脑缺血再灌注 ERK Caspase-3 |
Effects of 8 -opioid Receptor Agonists on Expressions of ERK Signaling Pathway and Caspase-3 Following Cerebral in Hippocampus of Rat |
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Institution: | HONG Miao-ling, HUANG Wei-qing, LIU Pei-yi, et alThe First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China |
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Abstract: | Objective: To observe the effects of δ -opioid receptor agonist DADLE ( D-AIa2, D-Leu5]enkephali ) on expression of ERK signaling pathway and Caspase-3 following cerebral ischemia-reperfusion in hippocampus of rat. Method: In total, 50 SD rats were randomly divided into 5 groups: a sham-operated, a I/R and three DADLE-treated ( different drug doses: 2 mg/kgA], 3 mg/kgB] and 5 mg/kgC] ) .Use improvement of two vessels occlusion add hypotension to establish the global cerebral ischemia/reperfusion model in rats.Injecting DADLE through external jugular vein after ischemic 15 min, and the rats were respectively killed after reperfusion 120rain.The expression of Caspase-3 were detected by Western blot, and the expression of non-phosphorylated ERK and phosphorylation of ERK ( P-ERK ) were detected by Immunohistochemistry assay. Result: The expression of ERK and P-ERK was lower in the Sham group than in the others ( P〈0.05 ), and compared with I/R group, the expression of ERK and P-ERK of each DADLE-treated group was higher obviously ( P〈0.05 ) . The expression of Caspase-3 in hippocampus was higher in the I/R group than in the Sham group, and compared with I/R group, the expression of Caspase-3 of each DADLE-treated group Was lower obviously ( P〈0.05 ) . Conclusion: DADLE may be have some protective effects on neurons in hippocampus after the global cerebral ischemia/reperfusion in rats, and it related. Shows that DADLE can play a role in protecting brain tissue by increasing the expression of ERK signaling pathway and inhibiting the expression of Caspase-3. |
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Keywords: | DADLE Global cerebral ischemia-reperfusion ERK Caspase-3 |
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