全自动血培养仪阳性病原菌种类及报警时间分析

目的：分析血培养阳性病原菌种类及仪器报警时间。方法：用BDBACTEC9120全自动血培养仪检测笔者所在医院2010年4月-2012年4月共计2180份血标本，分析血标本在需氧瓶和厌氧瓶中生长细菌的种类及仪器报警时间。结果：2180例血培养标本中报警阳性282例，其中真阳性263例，阳性率为12．1％。分离出细菌269株，其中革兰阳性球菌占40．1％，革兰阴性杆菌占57．2％，真菌占2．6％。其中仅需氧瓶报阳的阳性率为6．3％，仅厌氧瓶报阳的阳性率为2．8％，需氧瓶及厌氧瓶均报阳的阳性率为2．9％；最快阳性检出时间为5．02h，24h内检出的阳性数占69．1％，48h检出的阳性数占92．5％，72h检出的阳性数占97．O％；假阳性率为0．87％，假阴性率为O．09％。结论：应用BDBACTEC9120全自动血培养仪同时进行需氧瓶及厌氧瓶培养能提高血培养阳性率，缩短了阳性检出时间。

Analysis of the Positive Pathogenic Bacteria and the Alarm Time by Automated Blood Culture System

Objective： To analyse the positive pathogenic bacteria of the blood culture and the alarm time.Method： A total of 2180 blood specimens were collected from April 2010 to April 2012 in our hospital and the specimens were detected by BD BACTEC 9120 automated blood culture system.Analyse the bacterial species and alarm time of the blood specimens in aerobic and anaerobic bottles.Result： 282 blood culture positive samples were isolated from the 2180 blood culture samples, which was true positive in 263 eases.The positive rate of the blood culture was 12.1%, which 269 strains of pathogens were isolated, including Gram-positive cocci accounted for 40.1%, gram negative bacilli 57.2%, fungi 2.6%.However the positive rate was just 15.6% only from aerobic bottles and 5.7% only from anaerobic ones,and 20.9% from both of the two bottles.The fastest positive time was 5.02 h, during the test, the number of positive reactions accounted for 69.1% in 24 hours, 92.5% in 48 hours, and 97% in 72 hours.The false positive rate was 0.87%, and the false negative rate was 0.52%.Conclusion： Application of both aerobic and anaerobic cultivation detected by BD BACTEC 9120 automated blood culture system can improve the biood culture positive rate, shorten the positive detection time.