Memantine对新生大鼠缺氧缺血性脑损伤的影响

目的：研究美金胺(Memantine)对新生大鼠缺氧、缺血性脑损伤(HIBD)脑保护作用及机制。方法：应用新生7日龄Wistar大鼠随机分为假手术组、HIBD组、Memantine治疗组，采用结扎左侧颈总动脉并吸入8％O2 92％N2混合气体制成新生大鼠HIBD动物模型。于HIBD后即刻腹腔注射Memantine 20mg/kg或等量生理盐水，48h后处死，用放射免疫法测血清NSE水平，用硝酸还原酶法测血清NO水平．用H-E染色观察脑组织病理变化。结果：Memantine可使脑组织病理改变明显减轻，可降低血清中NSE浓度．可降低血清中NO浓度。结论：Memantine对脑缺氧缺血损伤具有脑保护作用；Memantine通过阻断NMDA受体减少毒性NO的生成，是其脑保护作用可能机制之一。

Effect of Memantine on hypoxic-ischemic brain damage in neonatal rats and its mechanism

Objective: To explore the effect of Memantine on hypoxic-ischemic brain damage(HIBD) in neonatal rats and its mechanism. Methods: The HIBD models were established with 7-day postnatal Wistar rats and divided into three groups,namely control group ,HIBD group and Memantine treated group.HIBD models were prepared by permanent ligation of left common carotid artery combined with a temporary systemic hypoxia (inhaling 8%O 2+92%N 2). Right after hypoxic-ischemic damage ,Memantine or normal saline solution was injected intraperitoneally(20mg/kg). 48 hours after hypoxic-ischemic damage, nitrate reductase method was used to measure serum nitric oxide (NO) contents .Values of serum neuron-specific enolase(NSE) were measured by radioimmunoassay. Hematoxylin and eosin stain(H-E) was used to observe the pathological changes in cerebral tissues . Results: Memantine significantly reduced the pathological changes in cerebral tissues. and lowered NSE contents and NO contents in serum. Conclusion: It is suggested that intraperitoneal administration of Memantine can protect against hypoxic-ischemic brain damage in neonatal rats.Memantine: inhibits production of toxic NO by blocking NMDA receptor,which is its possible protective mechanism for HIBD.