| 圆弧青霉提取物对枯草杆菌芽胞DNA重组效应 |
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| 引用本文： | 马远方,甄应中,周刚,徐岷,宋爱云,徐友梅,刘桂亭. 圆弧青霉提取物对枯草杆菌芽胞DNA重组效应[J]. 郑州大学学报(医学版), 1990, 0(2) |
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| 作者姓名： | 马远方 甄应中 周刚 徐岷 宋爱云 徐友梅 刘桂亭 |
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| 作者单位： | 河南医科大学食管癌研究所(马远方，甄应中，周刚，徐岷，宋爱云，徐友梅)，河南医科大学食管癌研究所(刘桂亭) |
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| 摘 要： | 从河南食管癌高发区林县粮食中分离出24株圆弧青霉,采用Raulin-Thom液体培养基培养,经三氯甲烷萃取后得棕黄色粉末状提取物。用枯草杆菌芽胞DNA重组试验检测其重组效应。结果表明:不加(S-9)活化系统16株提取物为阳性(64%),加S-9后24株皆为阴性,提示提取物中含有直接诱变物。
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| 关 键 词： | 食管癌 重组修复试验 圆弧青霉 霉菌毒素 检测 |
The study of rec-assay ot the extracts from P.Cyclopium on Bacillus subtilis spores |
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| Ma Yuanfang,Zhen Yingzhong,Zhou Gang,Xu Min,Song Aiyun,Xu Youmei,Liu Guiting. The study of rec-assay ot the extracts from P.Cyclopium on Bacillus subtilis spores[J]. Journal of Zhengzhou University: Med Sci, 1990, 0(2) |
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| Authors: | Ma Yuanfang Zhen Yingzhong Zhou Gang Xu Min Song Aiyun Xu Youmei Liu Guiting |
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| Affiliation: | Ma Yuanfang;Zhen Yingzhong;Zhou Gang;Xu Min;Song Aiyun;Xu Youmei;Liu Guiting Department of Esophageal Cancer Research |
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| Abstract: | 24 strains of Penicillum Cyclopium Westling were isolated from the grain inLin Xian County of Henan Province, the area of high incidence of esophageal cancer.The strains were inoculated in Raulin-Thom fluid medium. The brownpowdery extract was extracted with chlorofrom from the condensed culturedfluid. The extracts were tested for DNA-damaging ability in the Rec-assay byusing the recmbination-deficient mutant of Bacillus Subtilis M45 and the parentstrain H 17 with their spores. 16 of 24 strains of P. Cyclopium were positive inthis system without metabolic activation. 24 strains were negative with metabolic activation. |
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| Keywords: | esophageal cancer rec-assay Penicillum cyclopium Westling mycotoxin |
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