| 荷非小细胞肺癌裸鼠体内131I-17-AAG的分布及其抗癌潜力 |
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| 引用本文： | 孙晋,刘璐,吴清华,聂琦,高海林,杨泽萱,黄鹰.荷非小细胞肺癌裸鼠体内131I-17-AAG的分布及其抗癌潜力[J].中华肿瘤防治杂志,2010,17(3). |
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| 作者姓名： | 孙晋 刘璐 吴清华 聂琦 高海林 杨泽萱 黄鹰 |
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| 作者单位： | 东南大学临床医学院核医学技术研究所,江苏,南京,210009 |
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| 基金项目： | 国家自然科学基金(30470500); 江苏省普通高校研究生科研创新计划项目(CX09B_068Z) |
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| 摘 要： | 目的:探讨131I-17-丙基胺基-17-去甲氧基格尔德霉素(131I-17-AAG)在荷瘤裸鼠体内的生物学分布及其抗癌机制。方法:通过不同给药方式(131I-17-AAG间质给药或静脉给药、Na131I间质给药对照及竞争性显像),显像比较12只模型鼠体内分布情况,每只5.55MBq0.1mL。1周后处死取瘤,电镜和免疫组化检测细胞凋亡和Ki-67、Bcl-2蛋白表达情况,设空白对照组(n=3)。另取24只,分别通过间质或尾静脉注射131I-17-AAG(每只1.85MBq0.1mL),不同时间处死,取血并测主要组织脏器的放射性计数率值。结果:显像提示,间质注射131I-17-AAG瘤体内放射性浓聚并长时间滞留,体内分布实验进一步证实;尾静脉给药瘤体内存在一定放射性摄取,药物体内清除较快;Na131I注射后迅速排出体外,无瘤内滞留;竞争性显像示瘤内摄取降低。电镜观测给药组较对照组肿瘤增长抑制显著。Bcl-2和Ki-67阳性表达率131I-17-AAG给药组要低于对照组。不同给药组组间比较及与对照组比较差异有统计学意义,Pmax=0.004。结论:131I-17-AAG间质给药在瘤组织内有明确药代学分布和药效学...
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| 关 键 词： | 癌 非小细胞肺 同位素标记 小鼠 裸 |
Biodistribution and anti-cancer potential of 131I-17-AAG in NSCLC xenograft-bearing nude mice |
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| SUN Jin,LIU Lu,WU Qing-hua,NIE Qi,GAO Hai-lin,YANG Ze-xuan,HUANG Ying.Biodistribution and anti-cancer potential of 131I-17-AAG in NSCLC xenograft-bearing nude mice[J].Chinese Journal of Cancer Prevention and Treatment,2010,17(3). |
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| Authors: | SUN Jin LIU Lu WU Qing-hua NIE Qi GAO Hai-lin YANG Ze-xuan HUANG Ying |
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| Institution: | SUN+Jin,LIU+Lu,WU+Qing-hua,NIE+Qi,GAO+Hai-lin,YANG+Ze-xuan,HUANG+Ying+Nuclear+Medicine+Technology+Institution,Clinic+Medical+College+of+Southeast+University,Nanjing+210009,P.R.China |
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| Abstract: | OBJECTIVE:To study the biodistribution and therapeutic efficiency of 131I-17-AAG in human non-small cell lung cancer(NSCLC) xenografts in mice.METHODS:In vivo imaging was performed through 131I-17-AAG intratumoral injection or tail veil injection,Na131I intratumoral injection and the blocking imaging group,using 12 xenografts with dosage of 5.55 MBq in 0.1 mL solution per mouse.The mice were sacrificed and tumor tissues were separated.Examinations were done through electron microscopy for tumor cell apoptos... |
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| Keywords: | carcinoma non-small cell lung Isotope labeling mice nude |
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