海鞘醇类似物抗肿瘤活性的研究

目的探讨海鞘醇类似物002抗肿瘤活性。方法采用MTS法检测不同浓度的海鞘醇类似物对HepG2、A549和NIH3T3等细胞增殖的抑制作用;流式细胞、Annexin V-FITS/7-AAD等技术检测海鞘醇类似物对HepG2、A549和NIH3T3等细胞凋亡的影响。结果海鞘醇类似物对HepG2和A549的增殖有明显抑制作用,抑制率分别为73.67%和62.4%;海鞘醇类似物在浓度为2.0μg/ml时,引起HepG2和A549细胞的早期凋亡率分别为25.72%和25.46%。而对NIH3T3细胞影响不大。结论海鞘醇类似物对人肝癌和肺癌细胞的增殖有较强抑制作用,且能促进人肝癌和肺癌细胞的早期凋亡。

Study on Antitumor of Analog from SC

Objective To investigate the antitumor effects of 002 analog from SC.Methods The growth inhibition effects of 002 on cell lines HepG2,A549 and NIH3T3 cell in vitro were examined with MTS assay.Influences on HepG2,A549,NIH3T3 cell apoptosis of 002 were observed by flow cytometry and annexin V-fitc/7-AAD fluorescence staining.Results 002 obviously inhibited the growth of HepG 2 and A549 cells in a dose-effect relationship,the inhibitory rate reached 73.67% and 62.40%.It caused HepG 2 and A549 cells to apoptosis while no effect on NIH3T3.Conclusions Effects of 002 on different cells in vitro are different,but show potent inhibitory effect to HepG2 and A549 cells.