| 青蒿琥酯对腺样囊性癌NACC细胞凋亡和侵袭的影响 |
| |
| 引用本文： | 俞立平,农晓琳,李佳荃,胥航,朴智. 青蒿琥酯对腺样囊性癌NACC细胞凋亡和侵袭的影响[J]. 中国实验方剂学杂志, 2013, 19(9): 237-241 |
| |
| 作者姓名： | 俞立平 农晓琳 李佳荃 胥航 朴智 |
| |
| 作者单位： | 1. 广西医科大学口腔医学院口腔颌面外科,南宁,530021
2. 广西医科大学医学科学实验中心,南宁,530021 |
| |
| 基金项目： | 国家自然科学基金项目(30060082);教育部"春晖计划"科研启动基金(教外司留[2003]593-7);广西卫生厅重点科研项目(重200006,重200927);广西科学基金项目(2010GXNSFD013047, 桂科回0836013) |
| |
| 摘 要： | 目的:探究青蒿琥酯(ART)对人腭部小涎腺腺样囊性癌(NACC)细胞凋亡、侵袭能力的影响.方法:NACC细胞经ART 100 mg·L-1干预后,吖啶橙/溴化乙锭(AO/EB)染色观察细胞形态学变化;Transwell小室检测对照组及ART 25,50,100 mg·L-1组细胞侵袭力;免疫细胞化学法测定各组细胞胞浆中血管内皮生长因子(VEGF)的蛋白表达量;实时荧光定量聚合酶链反应(Real-time qPCR)测定各组细胞VEGF mRNA的表达.结果:①经AO/EB染色,与对照组相比,ART 100 mg·L-1组可明显观察到凋亡细胞(细胞形态不规则,核染色质固缩);②Transwell实验中,与对照组相比,ART 25,50,100mg·L-1组细胞跨膜数减少(P＜0.01);③细胞免疫化学结果显示,与对照组相比,ART 25,50,100,200 mg·L-各组胞浆中VEGF的蛋白阳性表达量显著降低(P＜0.01);④Real-time qPCR测得各ART组VEGF基因相对表达量均明显低于对照组(P＜0.01).结论:青蒿琥酯可诱导NACC细胞的凋亡,抑制其侵袭能力,下调其VEGF的表达.
|
| 关 键 词： | 青蒿琥酯 腺样囊性癌 凋亡 侵袭 血管内皮生长因子 |
| 收稿时间： | 2012-11-25 |
Effect of Artesunate on Apoptosis and Invasion of Novel Human Palatal Salivary Gland Cell Line of Adenoid Cystic Carcinoma in vitro |
| |
| YU Li-ping,NONG Xiao-lin,LI Jia-quan,XU Hang and PIAO Zhi. Effect of Artesunate on Apoptosis and Invasion of Novel Human Palatal Salivary Gland Cell Line of Adenoid Cystic Carcinoma in vitro[J]. China Journal of Experimental Traditional Medical Formulae, 2013, 19(9): 237-241 |
| |
| Authors: | YU Li-ping NONG Xiao-lin LI Jia-quan XU Hang PIAO Zhi |
| |
| Affiliation: | Department of Oral and Maxillofacial Surgery, College of Stomatology, Guangxi Medical University, Nanning 530021, China;Department of Oral and Maxillofacial Surgery, College of Stomatology, Guangxi Medical University, Nanning 530021, China;Department of Oral and Maxillofacial Surgery, College of Stomatology, Guangxi Medical University, Nanning 530021, China;Medical Science Research Center, Guangxi Medical University, Nanning 530021, China;Department of Oral and Maxillofacial Surgery, College of Stomatology, Guangxi Medical University, Nanning 530021, China;Department of Oral and Maxillofacial Surgery, College of Stomatology, Guangxi Medical University, Nanning 530021, China |
| |
| Abstract: | |
| |
| Keywords: | artesunate adenoid cystic carcinoma apoptosis invasion VEGF |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《中国实验方剂学杂志》浏览原始摘要信息 |
|
点击此处可从《中国实验方剂学杂志》下载全文 |
|
