Effects of podocin transfection on CD2AP distribution in HEK293 cells |
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Authors: | Yugen SHA Songming HUANG Aihua ZHANG Fei ZHAO Ronghua CHEN |
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Institution: | (1) Department of Nephrology, Affiliated Nanjing Children’s Hospital of Nanjing Medical University, Nanjing, 210008, China;(2) Institute of Pediatrics, Nanjing Medical University, Nanjing, 210029, China |
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Abstract: | The aim of this paper is to construct a podocin fluorescence expression vector and observe the effects of podocin transfection
on CD2AP distribution in HEK293 cells. The pGEMT-easy vector containing the full-length cDNA encoding human podocin was cloned
and digested with BamHI and XhoI. The digested full-length podocin was subcloned into pEGFP-C2. The constructed plasmids were transfected into HEK293 cells
and its effects on CD2AP distribution were observed by immunofluorescence. The pEGFP-NPHS2 expression vector was successfully
constructed and podocin exclusively located on the HEK293 cell membrane. After podocin transfection, CD2AP redistributed from
the perinucleus to the cytoplasm in HEK293 cells. It can be concluded that podocin can recruit CD2AP to redistribute from
the perinucleus to the cytoplasm in HEK293 cells.
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Translated from Journal of Nanjing Medical University (Natural Science), 2007, 27(6): 527–529, 533 译自: 南京医科大学学报(自然科学版)] |
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Keywords: | podocin genetic vectors CD2AP |
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