水通道蛋白4在感染性脑水肿大鼠脑组织中变化的意义

目的观察内毒素致幼年大鼠感染性脑水肿后脑组织水通道蛋白4(AQP4)表达的变化。方法将50只幼鼠随机分为内毒素组(40只)和对照组(10只)。建立内毒素致大鼠感染性脑水肿模型。于术后6、12、24、48 h处死动物,分别行脑组织含水量、血脑屏障通透率测定,以伊文思兰含量判定血脑屏障通透率,并应用免疫组织化学技术检测脑组织内AQP4表达水平。结果内毒素组脑组织含水量和伊文思兰(EB)水平明显高于对照组。在脂多糖注射后6 h时,幼鼠脑组织AQP4表达明显增加,12 h时达高峰,各时间点与对照组比较均有显著性差异(P<0.01)。结论感染性脑水肿后脑组织AQP4表达增加,且与脑组织含水量呈正相关。

Significance of Expression of Aquaporin- 4 in Inflammatory Brain Edema in Infant Rats

Objective To observe the expression of aquaporin-4(AQP4)in inflammatory brain edema by injecting endotoxin into infant rats.Methods Fifty infant rats were divided into endotoxin group(n=40) and control group (n=10).Firstly,brain edema model was established by injecting endotoxin into infant rats.The brain tissue water content and Evens blue content were detected at 6,12,24,48 h after injection,and immunohistochemistry was used to investigate AQP4 expression in brain tissue.Results Brain water content and Evens blue content were significantly higher in endotoxin group than that in control group.The level of AQP4 significantly increased at(6 h) after injecting endotoxin,the peak time of it was at 12 h.The differences between the level of AQP4 in endotoxin group and control group had statistic significance at each time point.Conclusions The level of AQP4 increased in inflammatory brain edema by endotoxin into infant rats.It has positive relationship with brain tissue water content.