| 反义硫代修饰寡核苷酸体外抗呼吸道合胞病毒感染的研究 |
| |
| 引用本文： | 俞海国,廉国利,杨锡强,赵晓东,赵长安. 反义硫代修饰寡核苷酸体外抗呼吸道合胞病毒感染的研究[J]. 重庆医科大学学报, 2003, 28(3): 264-266 |
| |
| 作者姓名： | 俞海国 廉国利 杨锡强 赵晓东 赵长安 |
| |
| 作者单位： | 重庆医科大学儿科学院免疫研究室,重庆,400014 |
| |
| 基金项目： | 国家自然科学基金项目 ( 3 0 0 70 3 3 1) |
| |
| 摘 要： | 目的：在感染呼吸道合胞病毒(RSV)的9HTE细胞中观察与RSVNS1基因和M2基因互补的反义硫代寡核苷酸的抗病毒活性。方法：直接观察RSV的致细胞病变作用，用MTT方法测定细胞存活率。结果：1．RSV感染复数（moi)在0.1以上时，培养5天后RSV感染的9HTE细胞全部死亡。2．我们设计的反义核酸能够提高感染细胞的存活率，且呈量效依赖关系。结论：反义硫代寡核苷酸具有较明显的抗病毒活性，为进一步研究新型抗RSV药物奠定了基础。
|
| 关 键 词： | 呼吸道合胞病毒 反义核酸 人气道上皮细胞 |
| 文章编号： | 0253-3626(2003)03-0264-03 |
| 修稿时间： | 2002-09-17 |
Antisense phosphorothioate oligodeoxynucleotide resistance to respiratory syncytial virus infection in vitro |
| |
| YU Haiguo,et al. Antisense phosphorothioate oligodeoxynucleotide resistance to respiratory syncytial virus infection in vitro[J]. Journal of Chongqing Medical University, 2003, 28(3): 264-266 |
| |
| Authors: | YU Haiguo et al |
| |
| Abstract: | Objective:To investigate the antiviral effects of antisense phosphorothioate oligodeoxynucleotide (AODN) in 9HTE infected with respiratory syncytial virus (RSV)in vitro.Methods:In this study,the antisense phosphorothioate oligodeoxynucleotide which complemented to genomic NS1 and M2mRNA of RSV was used to investigate antiviral activity in vitro.The cytopathic effect was observed and the cell survival rates were measured by MTT assay.Results:1.9HTE cell infected with RSV almost all died when the multiplicity of infection(moi)is above 0.1 after 5 days cell culture.2 The antisense phosphorothioate oligodeoxynucleotide could increase the cell survival rates in a dose dependent manner.Conclution:These studies indicate that the genomic NS1 gene and M2mRNA may play an important role in regulating RSV replication and AODN may have inhibitory activity on RSV replication.The results established the basis for further study on new drugs against RSV infection. |
| |
| Keywords: | Respiratory syncytial virus Antisense oligodeoxynucleotide 9HTE cell |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
