| 赫赛汀对HER-2/neu基因高表达鼻咽癌细胞株生长的影响 |
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| 引用本文： | 顾康生,管忠震,李志明,侯景辉,何丽蓉. 赫赛汀对HER-2/neu基因高表达鼻咽癌细胞株生长的影响[J]. 临床肿瘤学杂志, 2005, 10(3): 225-229 |
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| 作者姓名： | 顾康生 管忠震 李志明 侯景辉 何丽蓉 |
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| 作者单位： | 230022,安徽医科大学第一附属医院肿瘤内科;510060,广州,中山大学附属肿瘤医院内科 |
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| 摘 要： | 目的：探讨赫赛汀(Herceptin)对HER-2／neu基因高表达鼻咽癌细胞株生长的影响及机制。方法：分别采用MTT法、^3H-TdR细胞掺入法检测Herceptin对鼻咽癌细胞株CNE-22和CNE-2体外生长的影响；用流式细胞仪检测Herceptin对CNE-2Z细胞生长、细胞周期和凋亡的影响。结果：Herceptin可抑制CNE-2Z细胞的生长，引起细胞凋亡指数的增加。结论：Herceptin可抑制高表达HER-2／neu基因鼻咽癌细胞株的生长，并诱导细胞凋亡的发生。
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| 关 键 词： | 鼻咽癌 HER-2/neu基因 赫赛汀 |
| 文章编号： | 1009-0460(2005)03-0225-05 |
| 修稿时间： | 2004-07-10 |
The effect of Herceptin on the growth of nasopharyngeal carcinoma cell lines with HER-2/neu gene overexpression |
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| GU Kang-sheng,GUAN Zhong-Zhen,LI Zhi-ming,HOU Jing-hui,HE Li-rong. The effect of Herceptin on the growth of nasopharyngeal carcinoma cell lines with HER-2/neu gene overexpression[J]. Chinese Clinical Oncology, 2005, 10(3): 225-229 |
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| Authors: | GU Kang-sheng GUAN Zhong-Zhen LI Zhi-ming HOU Jing-hui HE Li-rong |
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| Affiliation: | GU Kang-sheng,GUAN Zhong-zhen,LI Zhi-ming,HOU Jing-hui,HE Li-rong. Dept of Medical Oncology,Cancer center of Sun Yat-sen University,Guangzhou 510060,China |
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| Abstract: | Objective:To study the effect and mechanism of Herceptin on the growth of nasopharyngeal carcinoma (NPC) cell lines with HER-2/neu gene overexpression.Methods:The effect of Herceptin on the growth of NPC CNE-2Z and CNE-2 cell lines in vitro was tested by MTT and ~3H-TdR incorporation, and the effect of the cell growth and cell cycle and apoptosis on NPC CNE-2Z cell line was assayed by flow cytometer.Results:Herceptin could inhibit the growth of CNE-2Z cell line, and induce cell apoptosis index to increase.Conclusion:Herceptin inhibited the growth of CNE-2Z cell lines with HER-2/neu gene overexpression, and induced cell (apoptosis.) |
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| Keywords: | Nasopharyngeal carcinoma HER-2/neu gene Herceptin |
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