Electrochemical Investigation of Curcumin–DNA Interaction by Using Hydroxyapatite Nanoparticles–Ionic Liquids Based Composite Electrodes |
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| Authors: | Merve Uca Ece Eksin Yasemin Erac Arzum Erdem |
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| Affiliation: | 1.Biotechnology Department, Graduate School of Natural and Applied Sciences, Ege University, 35100 Izmir, Turkey;2.Analytical Chemistry Department, Faculty of Pharmacy, Ege University, 35100 Izmir, Turkey;3.Pharmacology Department, Faculty of Pharmacy, Ege University, 35100 Izmir, Turkey; |
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| Abstract: | Hydroxyapatite nanoparticles (HaP) and ionic liquid (IL) modified pencil graphite electrodes (PGEs) are newly developed in this assay. Electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and cyclic voltammetry (CV) were applied to examine the microscopic and electrochemical characterization of HaP and IL-modified biosensors. The interaction of curcumin with nucleic acids and polymerase chain reaction (PCR) samples was investigated by measuring the changes at the oxidation signals of both curcumin and guanine by differential pulse voltammetry (DPV) technique. The optimization of curcumin concentration, DNA concentration, and the interaction time was performed. The interaction of curcumin with PCR samples was also investigated by gel electrophoresis. |
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| Keywords: | hydroxyapatite nanoparticles ionic liquid bionanomaterials nucleic acid interactions curcumin electrochemical biosensors |
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