运动性哮喘与中性粒细胞及白细胞介素-8关系的研究

目的探讨中性粒细胞与白细胞介素-8(IL-8)和运动性哮喘(EIA)的关系。方法2001-05-15—2005-12-19分别测定广东省佛山市第一人民医院呼吸内科EIA患者、非EIA患者和正常对照者运动前后周围血中性粒细胞超氧阴离子以及血浆和诱导痰中的IL-8,并进行比较分析。结果运动前EIA组与非EIA组中性粒细胞超氧阴离子略高于对照组,但差异无显著性,血浆及诱导痰中IL-8和MDA均高于对照组,其两组本身差异无显著性意义,但分别与对照组比较,差异均有显著性意义(P<0.05)。运动后(1)周围血中性粒细胞超氧阴离子水平EIA组与非EIA组较运动前均有明显提高,差异均有显著性意义(P<0.01),正常对照组无明显变化。(2)血浆及诱导痰中IL-8、MDA水平EIA组、非EIA组和正常对照组均高于运动前,EIA组、非EIA组各自与运动前比较差异有显著性(P<0.01),正常对照组与运动前比较差异无显著性意义(P>0.05)。结论中性粒细胞产生的氧自由基与白细胞介素-8在运动性哮喘的发生中起重要作用。

Study on the effect of neutrophil and interleukin-8 on exercise-induced asthma

Objective To explore the relationship of neutrophil and interleukin-8(IL-8)with exercise-induced asthma(EIA).Methods From May 15,2001 to Dec 19.2005 the levels of peripheral neutrophil superoxide anion and IL-8 in the blood and the induced sputum from patients with EIA,non EIA and normal control subjects were measured respectively before and after exercises,meanwhile a comparison was made among them.Results Before exercise:the level of neutrophil superoxide anion was slightly higher in EIA group and non EIA group than that in control group;however,the differences were insignificant;the IL-8 and MDA levels in blood and induced sputum were higher in EIA group and non EIA group than those in control group,but there was no significant difference between EIA group and non EIA group;however,they were significantly different between EIA group and control group,non EIA group and control group respectively( P <0.05).After exercise:(1)the levels of peripheral neutrophil superoxide anion were increasing significantly in both EIA group and non EIA group compared with those before exercise.The differences were significant(P<0.01).There was no significant change in control group.(2)The IL-8 and MDA levels in blood and induced sputum in EIA group,non EIA group and control group were higher than those before exercise;and significant differences were found in EIA group and non EIA group respectively(P<0.01)while the differences were insignificant in control group(P>0.05).Conclusion Oxyradical produced by neutrophil and IL-8 has significant effect on the production of EIA.