内淋巴囊的神经分布

目的:通过实验从形态学上直接验证内淋巴囊上存在神经纤维,为梅尼埃病的病因病理学研究提供新的思路。方法:使用成年SD大鼠15只,经活体心脏灌注,取双侧颞骨,常规石蜡包埋处理并切片。用免疫组织化学方法标记,确认抗体神经特异性烯醇化酶(neuronal specific enolase,NSE)、胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、神经微丝(neurofilament,NF)在内淋巴囊(Endolymphatic Sac,ES)上的表达。结果:在光学显微镜下,NSE、GFAP、NF一抗在内淋巴囊上皮及上皮下表达为棕黄色阳性着色颗粒。结论:内淋巴囊近侧端、中间段和远侧段均有神经纤维分布,以近侧段最为丰富。

Innervation of the rat endolymphatic sac

Objective: The endolymphatic sac(ES) is a part of the homeostasis-regulating system of the membranous labyrinth of the inner ear.Factors that influence blood flow in the sac may affect its function.This blood flow may be influenced by autonomic innervation;however,no such innervation has been demonstrated.The purpose of this study is to confirm the existence of the nerve fibres on the endolymphatic sac.Methods: Fifteen healthy Swards white rats were divided into three groups randomly.The temporal bones were taken out and were processed and sectioned by paraffin-embedded technique.The sections of ES were stained by the antibody that was not labeled by biotin including the antibody for neurone specific enolase(NSE), antibody for glial fibrillary acidic protein(GFAP) and antibody for neurofilament(NF).The slides were observed by light-microscope.Results: On light-microscope,the brown positive staining was observed in the epithelia of ES.However,there was not any positive staining on the negative control slides.Conclusion: Nerve fibers exactly exist in the epithelia of ES.