EGFR在甲状腺乳头状癌中突变及表达的临床病理分析

目的 探讨甲状腺乳头状癌(papillary thyroid carcinoma,PTC)表皮生长因子(epidermal growth factor receptor,EGFR)基因突变及其编码蛋白质表达的临床病理意义.方法 应用蝎形探针扩增阻遏突变系统(amplification refractory mutati...

Clinicopathological Significance of EGFR Gene Mutation and Its Encoding Protein Expression in Papillary Thyroid Carcinoma

Objective To investigate the clinicopathological significance of epidermal growth factor(EGFR)gene mutation and EGFR protein expression in papillary thyroid carcinoma(PTC).Methods Scorpion probe amplification repressor mutation system(ARMS)and immunohistochemistry were used to detect EGFR gene mutation and EGFR protein expression in 99 PTC tissues.The clinicopathological significance was analyzed correspondingly.Results The expression level of EGFR protein in PTC group with lymph node metastasis was higher than that in those without lymph node metastasis(P<0.05).There was no correlation between EGFR protein expression and tumor extrathyroid invasion,clinical stage and other parameters(P>0.05).No common mutation of EGFR gene Exon18,19,20,21 was found in 99 PTC cancer tissues,which was analogue to the research data of EGFR gene in PTC from the cancer genome atlas(TCGA)database.Conclusion The overexpression of EGFR protein in PTC tissue increases the lymph node metastasis rate of PTC.At the same time,the mutation of exon 18,19,20,21 of EGFR gene is not detected in the corresponding PTC tissue.Therefore,the targeted drugs against EGFR tyrosine kinase used in non-small cell lung cancer may not be the choice for the treatment of PTC.