血清25-羟维生素D3及总免疫球蛋白E水平与婴幼儿反复喘息的相关性研究

目的 探讨血清25-羟维生素D3 25-（OH）D3]及总水平与婴幼儿反复喘息的关系.方法 分别采用酶联免疫吸附测定、免疫比浊法,检测反复喘息的婴幼儿60例（反复喘息婴幼儿组）及正常对照儿童60例（对照组）血清中25-（OH）D3、TIgE含量.采用全自动血细胞分析仪检测2组外周血嗜酸性粒细胞百分比（EOS％）,并做比较.结果 反复喘息婴幼儿组血清25-（OH）D3含量明显低于对照组,2组比较差异有统计学意义（18±6）μg/L比（38 ±8） μg/L,t=15.81,P=0.000];反复喘息婴幼儿组血清TIgE含量、EOS％明显高于对照组,2组比较差异有统计学意义（122±18） IU/ml比（51±10） IU/ml,t=25.84,P=0.000;（3.02±1.82）％比（0.72±0.27）％,=9.73,P=0.000];反复喘息婴幼儿组血清25-（OH） D3含量与TIgE含量存在负相关（r=-0.783,P=0.000）,对照组血清25-（OH）D3含量与TIgE含量无相关性（r=-0.013,P=0.924）.2组血清25-（OH） D3含量与EOS％无相关性（r=-0.084,P=0.523;r=-0.137,P =0.298）.结论 血清25-（OH）D3的低下可能为婴幼儿反复喘息的高危因素;反复喘息的婴幼儿组血清25-（OH） D3与TIgE有明显负相关性,表明婴幼儿反复喘息与变态反应密切相关.补充维生素D3可能为临床预防婴幼儿反复喘息提供新思路.

Serum levels of 25 hydroxy vitamin D3 and total IgE in infants with recurrent wheezing

Objective To study the changes and clinical significance of serum levels of 25 hydroxy vitamin D3 25-（OH）D3] and total IgE and peripheral blood eosinophilia cell percentage （EOS%）in infants with recurrent wheezing.Methods Sixty infants with recurrent wheezing and sixty healthy children were enrolled.Enzyme linked immunosorbentassay was used to detect the levels of serum 25-（OH）D3 and immunoturbidimetry was used to detect the levels of total IgE.Automatic blood cell analyzer was used to detect EOS% of the two groups.Results Serum 25-（OH） D3 levels decreased significantly in infants with recurrent wheezing group,compared with the normal group （18 ±6）μg/L vs （38 ±8）μg/L] （t =15.81,P =0.000）.In contrast,serum total IgE levels increased significantly in infants with recurrent wheezing group,compared with the normal group （122 ± 18）IU/ml vs （51 ± 10）IU/ml,（3.02 ± 1.82）% vs （0.72 ±0.27）%] （t =25.84,P =0.000）.EOS% increased significantly in the infants with recurrent wheezing group compared with the normal group（t =9.73,P =0.000）.Serum 25-（OH）D3 levels were negatively correlated with serum total IgE level in the infants with recurrent wheezing group（r =-0.768,P =0.000）.There was no correlation between serum 25-（OH） D3 levels and serum total IgE levels in the normal group（r =-0.013,P =0.924）.Serum 25-（OH）D3 levels had no correlation with EOS% in both groups （r =-0.084,P =0.523;r =-0.137,P=0.298）.Conclusions Low serum25-（OH）D3 may be the risk factor of recurrent wheezing in infants.Serum 25-（OH）D3 levels are negatively correlated with serum total IgE levels in the infants with recurrent wheezing group; recurrent wheezing in the infants is closely related to allergic reaction.The supplement of vitamin D3 may provide new ideas for clinical prevention of infant recurrent wheezing.