| 鼻咽癌的单克隆抗体研究:杂交瘤细胞系的建立及初步鉴定 |
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| 引用本文： | 孙去病,郭敏,李小玲,肖家声,潘世宬. 鼻咽癌的单克隆抗体研究:杂交瘤细胞系的建立及初步鉴定[J]. 中南大学学报(医学版), 1987, 0(1) |
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| 作者姓名： | 孙去病 郭敏 李小玲 肖家声 潘世宬 |
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| 作者单位： | 湖南医学院肿瘤研究室(孙去病，郭敏，李小玲，肖家声)，湖南医学院肿瘤研究室(潘世宬) |
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| 摘 要： | 用鼻咽癌细胞株 CNE-1作抗原,免疫 Balb/c 小鼠,取脾细胞与骨髓瘤细胞融合,获得13株杂交瘤细胞系,用酶联免疫吸附试验,杂交瘤 H_(13)分泌的单克隆抗体显较好的特异性,用间接免疫荧光检查,这些抗体作用部位为癌细胞膜上的肿瘤相关抗原。
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| 关 键 词： | 单克隆抗体 鼻咽肿瘤 酶联免疫吸附 间接免疫荧光 |
MONOCLONAL ANTIBODY AGAINST NASOPHARYNGEAL CARCINOMA:ESTABLISHMENT OF HYBRID CELL LINES AND PRELIMINARY CHARACTERIZATION |
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| Sun Qibing,Guo Ming,Li Xiaolin,Xiao Jiasheng,Pan Shichen Cancer Research Laboratory,Hunan Medical College. MONOCLONAL ANTIBODY AGAINST NASOPHARYNGEAL CARCINOMA:ESTABLISHMENT OF HYBRID CELL LINES AND PRELIMINARY CHARACTERIZATION[J]. Journal of Central South University. Medical sciences, 1987, 0(1) |
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| Authors: | Sun Qibing Guo Ming Li Xiaolin Xiao Jiasheng Pan Shichen Cancer Research Laboratory Hunan Medical College |
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| Abstract: | Mouse myeloma cells were fused with spleen cells from Balb/c mice immunizedwith the CNE-1 human nasopharyngeal carcinoma cell line.Among hybridomas,thirteen were selected and cloned.Monoclonal antibody secreted by hybridoma H13show relative specificity to target cells by ELISA.In immunofluorescent assayall monoclonal antibodies reacted with membrance associated antigens of carcinomacells. |
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| Keywords: | Monoclonal Antibody Nasopharyngeal Carcinoma Fluorescentantihbody technic Enzyme-linked Immunosorbent Assay |
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