| FSGS患者尿蛋白对肾小管上皮细胞活力及转分化的影响 |
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| 引用本文： | 周宏久,吴丽丹,陶静莉,杨陈,刘华锋.FSGS患者尿蛋白对肾小管上皮细胞活力及转分化的影响[J].中国中西医结合肾病杂志,2009,10(9):761-764,I0002. |
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| 作者姓名： | 周宏久 吴丽丹 陶静莉 杨陈 刘华锋 |
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| 作者单位： | 1. 广东医学院附属跃院肾病研究所,湛江,524001
2. 广东医学院附属跃院肾病研究所,湛江,524001;广东省东莞市石龙人民医院肾内科,东莞,511700 |
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| 基金项目： | 广东省自然科学基金资助项目(No.815240231000014) |
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| 摘 要： | 目的：探讨局灶性-节段性肾小球硬化症（focal segmental glomerulosclerosis，FSGS）患者尿蛋白对肾小管上皮细胞（TECs）活力及转分化的影响。方法：用硫酸铵沉淀法提取FSGS患者尿液中的总蛋白成分，应用不同浓度尿蛋白处理体外培养人近端肾小管上皮细胞，然后应用全自动生化分析仪检测不同浓度尿蛋白刺激后HK-2细胞LDH值并计算其释放率、免疫细胞化学（ICC）法检测不同浓度尿蛋白刺激后HK-2细胞E-钙黏蛋白（e—cadherin）的表达水平和Western blotting法检测HK-2细胞α-平滑肌肌动蛋白（Q—SMA）蛋白表达水平。结果：所提取的尿蛋白主要成分为白蛋白、转铁蛋白和IgG等，分别占59．3％、15．3％和13．8％；肾小管上皮细胞LDH释放率和α—SMA蛋白的表达水平随尿蛋白浓度的升高而升高；E—cadherin的表达水平随着尿蛋白浓度的升高而下降。结论：FSGS尿蛋白可通过诱导肾小管上皮细胞损伤和转分化而促进肾小管-间质纤维化。
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| 关 键 词： | 尿蛋白 肾小管上皮细胞 活力 转分化 |
The Effects of Urine Protein from Patients with FSGS on Cell Vitality and Transdifferentiation in Renal Tubular Epithelial Cells |
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| ZHOU Hongjiu,WU Lidan,TAO Jingli,et alInstitute of Nephrology,Affiliated Hospital of Guangdong Medical College,Zhanjiang.The Effects of Urine Protein from Patients with FSGS on Cell Vitality and Transdifferentiation in Renal Tubular Epithelial Cells[J].Chinese Journal of Integrated Traditional and Western Nephrology,2009,10(9):761-764,I0002. |
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| Authors: | ZHOU Hongjiu WU Lidan TAO Jingli Institute of Nephrology Affiliated Hospital of Guangdong Medical College Zhanjiang |
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| Abstract: | Objective:To explore the effects of urine protein from patients with FSGS on vitality and transdifferentiation in renal tubular epithelial cell(TECs).Methods:Urine protein was abstracted by fractional precipitation method with ammonium sulfate from nephrotic syndrome patients with FSGS.HK-2 cells were stimulated with urine protein in different concentration.The releasing rate of Lactic acid dehydrogenase(LDH)of HK-2 cells was measured by automatic biochemistry analyzer.The expression of E-cadherin in HK-2 c... |
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| Keywords: | Urine protein Renal tubular epithelial cells Vitality Transdifferentiation |
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