肝细胞癌组织中转化生长因子α表达及其与乙型肝炎病毒感染的 …

目的 研究肝细胞（ＨＣＣ）中转化生长因子α（ＴＧＦα）的表达及其与ＨＢＶ感染的关系。方法 应用原位杂交及免疫组化ＳＰ法对５３例ＨＣＣ及１４例正常肝组织吕ＴＧＦαｍＲＮＡ、ＴＧＦα蛋白、ＨＢＶ ＤＮＡ进行检测。结果 ５３例ＨＣＣ组织中ＴＧＦαｍＲＮＡ、ＴＧＦα蛋白的阳性率分别为３０．２％（１６例）、７３．６％（３９例）,显著高于无表达的正常对照组（Ｐ〈０．０５）。癌旁不典型增生肝细胞组ＴＧＦαｍＲＮ

Expression of transforming growth factor alpha and its relationship with HBV infection in hepatocellular carcinomas

OBJECTIVE: To assess the expression of TGF alpha in hepatocellular carcinoma and its relationship with HBV infection. METHODS: Using in situ hybridization and streptavidin-peroxidase immunohistochemistry to detect TGF alpha mRNA, TGF alpha protein and HBV DNA in 53 cases of HCC and 14 cases of controls. RESULTS: Positive rates of TGF alpha mRNA and TGF alpha protein in carcinoma tissue were 30.2% and 73.6% respectively, which were higher than in normal controls (P < 0.05). The expression of TGF alpha mRNA and TGF alpha protein was increased significantly in dysplastic liver cells (DLC) locating aside from the tumor tissue in comparing with those of non-dysplastic liver cells (NDLC) (P < 0.05). Positive rates of HBV DNA in carcinoma tissue and paratumorous liver tissue were 56.6% and 60.4% respectively, which were higher than in the normal controls (P < 0.001). Positive rate of HBV DNA in the nuclei of tumor tissues, tissue of the dysplastic areas and non-dysplastic areas was lowering down successively (P < 0.05). There was a significant relationship between expression of TGF alpha mRNA and TGF alpha protein in the carcinoma tissues and HBV DNA in the tumorous and its surrounding tissues respectively. CONCLUSIONS: Expression of TGF alpha in carcinoma tissues was closely associated with the existence of HBV DNA and TGF alpha may participate in the early stage of liver carcinogenesis. The increased expression of HBV DNA in nuclei may serve as an important morphology marker in distinguishing carcinoma from DLC and NDLC.