乙型肝炎病毒前S1抗原检测的临床应用价值

目的 探讨乙型肝炎病毒前S1抗原与病毒复制的相关性.方法 采用酶联免疫吸附试验(ELISA)和荧光定量(PCR)检测100例乙型肝炎患者血清的前S1抗原、乙型肝炎病毒e抗原(HBeAg)和乙型肝炎病毒脱氧核糖核酸(HBV-DNA).结果 100例乙型肝炎患者标本中,前S1抗原78例阳性,阳性率为78%;HBV-DNA 86例阳性,阳性率为86%;HBeAg 56例阳性,阳性率为56%,在56例HBeAg阳性中,前S1抗原阳性例数为44例,阳性率为78%,HBV-DNA的阳性例数为49例,阳性率为88%,前S1抗原与HBV-DNA的差异性用χ2检验差异无统计学意义(P>0.05).在86例HBV-DNA阳性中,前S1抗原阳性例数为74例,阳性率为86%,HBeAg阳性例数为50例,阳性率为58%,前S1抗原与HBeAg的检测结果用χ2检验差异有统计学意义(P<0.05).结论 乙型肝炎病毒前S1抗原与HBV-DNA高度相关,是乙肝病毒在体内复制的可靠指标.

Clinical application value of pre-S1 antigen detection for hepatitis B virus

Objective To investigate the relativity between pre-S1 antigen of hepatitis B virus with viral replication. Methods ELISA and the fluorescence quantitative PCR were used to detect the pre-S1 antigen level,HBeAg and HBV-DNA in 100 cases of hepatitis B. Results Among 100 samples in 100 cases of hepatitis B,pre-S1 antigen was positive in 78 cases（78%）,HBV-DNA was positive in 86 cases（86%） and HBeAg was positive in 56 cases（56%）.Among 56 cases of positive HBeAg,pre-S1 was positive in 44 cases（78%） and HBV-DNA was positive in 49 cases（88%）,showing that no statistical difference existed between pre-S1 and HBV-DNA（P0.05）.Among 86 cases of positive HBV-DNA,pre-S1 antigen was positive in 74 cases（86%） and HBeAg was positive in 50 cases（58%）,showing statistical difference between pre-S1 antigen and HBV-DNA（P0.05）. Conclusion PreS1 is highly related with HBV-DNA,which is a reliable indicator for in vivo replication of hepatitis B virus.