依布硒啉对急性肺损伤大鼠氧化应激的影响

目的 观察依布硒啉对内毒素性急性肺损伤的保护作用.方法 健康雄性SD大鼠,随机分成6组:正常对照组、模型组、依布硒啉高剂量组、中剂量组、低剂量组和地塞米松对照组.通过尾静脉注射LPS(5 mg/kg)建立模型,治疗组于造模前30 min,大鼠腹腔注射依布硒啉(7.5 mg/kg、15 mg/kg、30 mg/kg),对照组和模型组分别注入等量溶剂.造模后6h,麻醉放血处死动物,并取肺组织,测定肺湿质量/干质量.采用硫代巴比妥酸和黄嘌呤氧化法分别检测肺组织中超氧化物歧化酶SOD活性和丙二醛MDA及髓过氧化物酶(MPO)含量.光镜下观察肺组织病理学改变.结果 依布硒啉治疗组与模型组相比,肺湿质量/干质量降低,肺组织SOD的活性明显增高,MDA和MPO含量显著降低(P＜0.05),病理改善明显.结论 依布硒啉对内毒素性急性肺损伤有一定保护作用,其机制可能与提高抗氧化能力有关.

The effect of Ebselen on oxidative stress in acute lung injury rats

Objective To observe the protective effect of ebselen on oxidative stress in endotoxin-induced acute lung injury.Methods Healthy male SD rats were randomly divided into six groups:normal control group(C group),model group(LPS),ebselen low-dose group(EbsL group),middle dose group(EbsM group),high-dose group(EbsH group) and dexamethasone(DEX group).By intravenous injection of LPS(5 mg/kg) model,the treatment group in modeling the first 30 minutes,rats and ebselen(7.5 mg/kg,15 mg/kg,30 mg/kg),dexamethasone(5 mg/kg),normal control group and model group were injected into the same amount of solvent.6 hours after modeling,the animals were anesthetized and bleeding killed.The lung tissue were collected to detect W/D and the levels of SOD、MDA and MPO in lung homogenates and the change of light microscopy of lung histopathology.Results Ebselen treatment group compared to model group,the W/D were significantly reduced;the levels of SOD in lung tissue were significantly increased(P<0.05);the levels of MDA and MPO in lung tissue were significantly decreased(P<0.05);the pathology improved significantly.Conclusion Ebselen has a protective effect on endotoxin induced acute lung injury and its mechanism related with the inhibition of oxidative stress.