| PCR检测技术在志贺菌引起的腹泻疫情中的应用 |
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| 引用本文： | 贺电,张晓曦,单志兰,曾照丽,白瑞婷,王晨,韩庆华. PCR检测技术在志贺菌引起的腹泻疫情中的应用[J]. 中国卫生检验杂志, 2007, 17(5): 858-860 |
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| 作者姓名： | 贺电 张晓曦 单志兰 曾照丽 白瑞婷 王晨 韩庆华 |
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| 作者单位： | 北京市朝阳区疾病预防控制中心,北京,100021 |
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| 基金项目： | 朝阳区组织部优秀青年知识分子人才资助项目 |
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| 摘 要： | 目的：通过采用PCR方法对某次疫情中分离的福氏1 a志贺菌进行保守基因检测及毒力基因分析研究,探讨在感染性腹泻疫情中,PCR技术快速检测志贺菌的应用。方法：对标本中分离的7株福氏1 a志贺菌,用PCR方法检测侵袭性质粒抗原基因（ipaH）、志贺肠毒素1（ShET-1）和志贺肠毒素2（ShET-2）的基因检测,并进行基因分型。结果：7株F1 a菌均检测出ipaH基因,ShET-1和ShET-2,基因型为ShET1（＋）/ShET2（＋）,整个实验可在3 h内完成。结论：该所建立的PCR检测方法准确、快速、简便、特异性强,为其应用于环境、食品及临床腹泻样本中志贺菌的检测打下良好基础。ShET-1/ShET-2基因分型对福氏志贺菌暴发流行中的同源性分析有重要意义。
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| 关 键 词： | 志贺菌 快速检测 志贺肠毒素 |
| 文章编号： | 1004-8685（2007）05-0858-03 |
| 收稿时间： | 2007-01-12 |
| 修稿时间： | 2007-01-12 |
Application of PCR detecting technique for rapid diagnosis of bacillary dysentery |
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| He Dian, Zhang Xiao-xi. Application of PCR detecting technique for rapid diagnosis of bacillary dysentery[J]. Chinses Journal of Health Laboratory Technology, 2007, 17(5): 858-860 |
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| Authors: | He Dian Zhang Xiao-xi |
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| Affiliation: | Chaoyang Center for Disease Control and Prevention,Beijing 100021 ,China |
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| Abstract: | Objective:To explore the PCR rapid detection of Shigella spp.in bacillary dysentery by PCR assay of the conserved gene and toxin gene of the Shigella flexneri 1a isolated from a dysentery samples.Methods:Seven strains isolated from the dysentery were detected by PCR technique with the invasive plasmid antigen H(ipaH) gene,enterotoxin 1(ShET-1) and 2(ShET-2).Results:All the strains were ipaH positive and were found to produce ShET-1 and ShET-2.The assay could be finished in 3 hours.Conclusion:The PCR detection of ipaH,ShET-1 and ShET-2 gene is a simple and rapid method with protential application in the identification Shigella spp.in the environment,food and clinical samples. |
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| Keywords: | PCR |
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