Rapid detection of chimeric bcr/abl mRNAs in acute lymphoblastic and chronic myeloid leukemia by the polymerase chain reaction |
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Authors: | Juergen Maurer Eckhard Thiel |
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Affiliation: | (1) Department of Hematology and Oncology, Universitätsklinikum Steglitz, Free University of Berlin, Hindenburgdamm 30, W-1000 Berlin 45, Federal Republic of Germany |
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Abstract: | Summary We have developed a rapid method for the detection of bcr/abl mRNAs, the products of the BCR/ABL fusion genes. The method is based on the polymerasechain-reaction (PCR). Through the use of additional internal primers it is possible to detect directly a single Ph1-positive cell among 105 unaffected cells thus omitting time-consuming blotting procedures. The whole analytical procedure starting from RNA isolation to agarose gel electrophoresis including two rounds of PCR can be performed in less than six hours.Supported by the Wilhelm Sander-Stiftung, Neustadt/Donau and the Deutsche Krebsgesellschaft, Landesverband Berlin |
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Keywords: | Polymerase chain reaction Acute lymphoblastic leukemia Chronic myelogenous leukemia Philadelphia chromosome |
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