Partially hydrolyzed guar gum alleviates small intestinal mucosal damage after massive small bowel resection along with changes in the intestinal microbiota |
| |
| Authors: | Takayuki Fujii Yoichi Chiba Haruyuki Nakayama-Imaohji Shun Onishi Aya Tanaka Hiroto Katami Tatsuru Kaji Satoshi Ieiri Takanori Miki Masaki Ueno Tomomi Kuwahara Ryuichi Shimono |
| |
| Affiliation: | 1. Department of Pediatric Surgery, Faculty of Medicine, Kagawa University, Ikenobe, Mikicho, Kitagun, Kagawa, Japan;2. Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, Ikenobe, Mikicho, Kitagun, Kagawa, Japan;3. Department of Molecular Microbiology, Faculty of Medicine, Kagawa University, Ikenobe, Mikicho, Kitagun, Kagawa, Japan;4. Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University, Sakuragaoka, Kagoshima City, Japan;5. Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Ikenobe, Mikicho, Kitagun, Kagawa, Japan |
| |
| Abstract: | PurposeShort bowel syndrome is associated with intestinal mucosal inflammation and microbial dysbiosis, leading to intractable complications. Partially hydrolyzed guar gum (PHGG) has trophic and anti-inflammatory effects on the intestine. We investigated whether PHGG ameliorates small intestinal mucosal damage and alters the intestinal microbiota using a rat small bowel resection (SBR) model.MethodsSprague Dawley rats were divided into sham operation (Sham), Sham/PHGG, SBR, and SBR/PHGG groups. On day 21, all rats were euthanized. To assess small intestinal mucosal damage, the degeneration rate was morphometrically evaluated and immunohistochemically examined using anti-CD45 antibodies. Analyses of fecal microbiota using 16S rRNA and short-chain fatty acid production were also performed.ResultsThe mucosal degeneration rate was significantly higher in the SBR group than in the Sham or SBR/PHGG groups. The number of CD45-positive cells was significantly higher in the SBR group than in the Sham, Sham/PHGG, or SBR/PHGG groups. The relative abundance of family Lachnospiraceae was significantly higher in the SBR/PHGG group than in the SBR group.ConclusionsPHGG administration alleviated small intestinal mucosal damage which could be associated with modulation of the intestinal microbiota. |
| |
| Keywords: | Partially hydrolyzed guar gum Dietary fiber Short bowel syndrome Intestinal failure Dysbosis Short chain fatty acids |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
