丙泊酚对重症急性胰腺炎相关肺损伤保护作用的实验研究

目的：探讨丙泊酚对大鼠重症急性胰腺炎（SAP）相关肺损伤的保护作用及机制。方法45只雄性SD大鼠随机分为3组（n=15）：假手术组（S组）、重症胰腺炎组（SAP组）、丙泊酚组（P组）。以4％牛磺胆酸钠逆行胰胆管注射的方法制作SAP模型。丙泊酚组（P组）在制作SAP模型前30分钟经股静脉注射5mg·kg^-1，随后以10mg·kg^-1·h^-1。继续持续输注。各组均于造模12小时后取材，测定血清肿瘤坏死因子-a（TNF-ct）含量；采用RT-PCR法检测肺组织细胞间粘附分子-1（ICAM-1）mRNA的表达；同时检测肺组织丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性、湿干重比及对肺脏进行病理学评分。结果与SAP组相比，P组肺组织病理损伤程度、湿／干重比、肺组织MDA含量和SOD活性以及血清TNF—a和ICAM-1mRNA水平表达均显著降低（P〈0．05）。结论丙泊酚可减轻大鼠SAP相关肺损伤．其机制可能与其减少炎症因子的释放，减少中性粒细胞在肺内的积聚，抑制过氧化损伤有关。

Protective effects of propofol on severe acute pancreatitis associated lung injury

Objective To study the protective effects of propofol on the severe acute pancreatitis (SAP) associated lung injury in rats.Methods 45 male SD rats were randomly divided into three groups (n=15),sham operation group (S group),severe acute pancreatitis group (SAP group) and propofol group (P group).SAP models were established by retrograde injection of 4% sodium taurocholate into biliopancreatic duct.The levels of serum tumor necrosis factor-α (TNF-α) were measured in 12 h after model establishment.Expression of intercellular adhesion molecule-1 (ICAM-1) mRNA was detected by RT-PCR method.The levels of Maleic Dialdehyde (MDA) and Superoxide Dismutase (SOD) and Wet/Dry rate were measured in the lung tissues at the same time.The pathological changes of the lungs were observed as well.Results The pathological damage,wet/dry rate,the levels of MDA and SOD in lung tissues and the levels of serum TNF-α and expression of ICAM-1 mRNA were significantly lower in group P than those in group SAP (P<0.05).Conclusion Propofol may attenuate the SAP associated lung injury in rats partly by reducing the release of cytokine,decreasing the accumulation of neutrophil in the lung,and inhibiting peroxidized injury.