| 来源于浙江尖吻蝮蛇毒抗肿瘤多肽的纯化与特性 |
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| 引用本文： | 吴勇,徐宏江,张喜全,冯军.来源于浙江尖吻蝮蛇毒抗肿瘤多肽的纯化与特性[J].中国医药工业杂志,2012,43(6):428-431. |
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| 作者姓名： | 吴勇 徐宏江 张喜全 冯军 |
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| 作者单位： | 1. 中国医药工业研究总院上海医药工业研究院,创新药物与制药工艺国家重点实验室,上海200040
2. 江苏正大天晴药业股份有限公司,江苏南京,210038 |
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| 基金项目： | 微生物药物技术创新与新药创制产学研联盟(2010ZX09401-403) |
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| 摘 要： | 采用超滤和反相色谱法从浙江尖吻蝮蛇毒中分离出3个抗肿瘤活性多肽.结构研究表明它们分别为焦谷氨酰赖氨酰色氨酸(pyroGlu-Lys-Trp)、焦谷氨酰门冬酰胺酰色氨酸(pyroGlu-Asn-Trp)和焦谷氨酰谷氨酰胺酰色氨酸(pyroGlu-Gln-Trp).MTT试验结果显示,在10 μg/ml时,活性多肽对人低分化胃癌细胞BGC-283、乳腺癌细胞MDA-MB-231及人肝癌细胞SMMC-7721有30％～60％的增殖抑制活性.
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| 关 键 词： | 蛇毒 多肽 纯化 抗肿瘤活性 |
Purification and Characterization of Anti-tumor Peptides from Venom of Zhejiang Agkistrodon acutus |
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| Authors: | WU Yong XU Hongjiang ZHANG Xiquan FENG Jun |
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| Institution: | 1(1.State Key Lab.of New Drug and Pharmaceutical Process,Shanghai Institute of Pharmaceutical Industry,China State Institute of Pharmaceutical Industry,Shanghai 200040;2.Chia-tai Tianqing Pharmaceutical Co.,Ltd.,Nanjing 210038) |
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| Abstract: | Three peptides with anti-tumor activity were purified by ultrafiltration and RP-HPLC from the venom of Zhejiang Agkistrodon acutus.The studies of structure indicated that they were pyroglutamyl lysyl tryptophane(pyroGlu-Lys-Trp),pyroglutamyl asparagyl tryptophane(pyroGlu-Asn-Trp) and pyroglutamyl glutaminyl tryptophane(pyroGlu-Gln-Trp),respectively.The results of MTT screening assays showed that these peptides performed 30%-60% activity on the inhibitory of proliferation of human poorly differentiated gastric cancer cells BGC-823,breast cancer cells MDA-MB-231 and hepatoma cells SMMC-7721 at 10 μg/ml. |
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| Keywords: | snake venom polypeptide purification anti-tumor activity |
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