不平衡氨基酸对肿瘤生长的影响

目的 比较几种不平衡氨基酸营养支持对肿瘤生长的影响。方法 荷Walker-256癌肉瘤SD大鼠，空肠喂饲肠外营养制剂10天，据所喂饲制剂中氨基酸组成的不同，分为：A组（平衡氨基酸组）、B组（增量精氨酸不平衡氨基酸组）、C组（去蛋氨酸不平衡氨基酸组）、D组（去缬氨酸不平衡氨基酸组）、E组（增量精氨酸、去蛋氨酸、去缬氨酸不平衡氨基酸组）。肿瘤体积增长速度、肿瘤重量、肿瘤重量／尸重，肿瘤组织PCNA指数，肿瘤细胞周期各时相百分比以及DNA倍性作为观察肿瘤生长速度的指标。结果 上述各指标值显示：B、C、D组与A组相比，除DNA倍性外，其余各项指标值均降低（P＜0．01），但B、C、D组间比较无显性差异（P＞0．05）。E组与B、C、D组相比较，所有指标值均显降低（P＜0．05）。结论 平衡氨基酸促进肿瘤生长；增量精氨酸、去蛋氨酸、去缬氨酸三种不平衡氨基酸对肿瘤生长有同等抑制作用；增量精氨酸、去蛋氨酸、去缬氨酸复合不平衡氨基酸对肿瘤生长抑制作用更显。

Influence of imbalance amino acids on tumor growth in tumor-bearing rats

Objective To study effects of imbalance amino acids nutritionsupport on tumor growth in tumor-bearing rats. Methods Parenteral nutritional solution had been fed for 10 days by jejumostomy in rats bearing Walker-256 carcinosarcoma. The tumor-bearing rats were divided into five different groups according to formulas of amino acids in parenteral nutritional solution respectively: group A (balance amino acid), group B (arginine-enriched amino acid), group C (methionine-depleted amino acid), group D (valine-depleted amino acid) and group E (complex amino acids of arginine-enriched、methionine depleted and valine-depleted) . Indices of tumor growth: tumor volume、tumor weight、tumor weight/carcass body weight、the expression of proliferating cell nuclear antigen (PCNA)、DNA ploidy and cell cycle of tumor tissue were investigated. Results The levels of above indice except DNA ploidy were as follows: group B、C and D were significantly lower than group A (P<0.01), but there were no significant difference among group B、C and D (P>0.05). All tumor growth indices of group E were significantly less than those of group B、C and D (P<0.05). Conclusions Balance amino acid stimulates tumor growth;Arginine enriched、methionine-depleted and valine-depleted amino acids inhibit tumor growth; Complex amino acids of arginine-enriched、methionine-depleted and valine-depleted have stronger inhibitory effect on tumor growth.